Recombinant Human LCE3C Protein: FAQs & Key Facts

Recombinant Human LCE3C Protein is a recombinant form of the human Late Cornified Envelope 3C protein, a small structural component of the epidermal cornified cell envelope associated with skin barrier integrity and implicated in inflammatory skin conditions such as psoriasis.

Product Name

Recombinant Human LCE3C Protein

SKU

rp369053

Protein Family

Late Cornified Envelope (LCE) protein family, epidermal differentiation complex

Biological Origin

Human (Homo sapiens)

Primary Research Application

Skin barrier research, psoriasis and inflammatory skin disease studies, epidermal differentiation studies

Supplier

Aladdin Scientific

What Is LCE3C and Why Is It Scientifically Relevant?

LCE3C (Late Cornified Envelope Protein 3C) belongs to the LCE gene cluster located within the Epidermal Differentiation Complex (EDC) on human chromosome 1q21. Members of the LCE protein family are small, proline- and glutamine-rich structural proteins that are incorporated into the cornified cell envelope (CE) during the terminal differentiation of keratinocytes. The CE is an insoluble protein scaffold beneath the plasma membrane of corneocytes that is essential for the skin barrier function.

LCE3C has attracted particular scientific interest because deletions of the LCE3B and LCE3C genes (collectively referred to as the PSORS4 locus) have been associated with susceptibility to psoriasis, a chronic inflammatory skin disorder. Recombinant LCE3C protein therefore serves as a valuable tool for researchers investigating keratinocyte biology, epidermal barrier formation, and the molecular mechanisms underlying psoriasis and atopic dermatitis.

Common Questions About Product Specifications

What is the source organism? Recombinant Human LCE3C Protein is derived from the human (Homo sapiens) LCE3C gene sequence.

What expression system is used? Expression system details for SKU rp369053 should be confirmed directly with Aladdin Scientific or on the product datasheet, as this information was not fully specified in the available source material. Common systems for small structural proteins include E. coli, yeast, or cell-free expression platforms.

What purity level can be expected? Purity and endotoxin specifications are provided on the official product datasheet. Researchers are advised to download or request the current certificate of analysis (CoA) for lot-specific data before use in sensitive assays.

What formulation and concentration are provided? Formulation details including buffer composition, protein concentration, and additives are lot-specific and available on the product CoA. Contact Aladdin Scientific technical support for the most current specifications.

Storage, Handling, and Stability Guidance

Recombinant proteins require careful storage to maintain activity and integrity. As a general guideline for recombinant proteins of this class:

• Short-term storage (up to 1 month): Store at 4 °C in the supplied buffer.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Avoid repeated freeze-thaw cycles: Prepare single-use aliquots upon first thaw.
• Carrier protein: If the protein will be stored in dilute form, consider adding a carrier protein such as 0.1% BSA to prevent adsorption losses.

Always refer to the product-specific datasheet for validated storage recommendations, as formulation details may vary between lots. For confirmed storage conditions for SKU rp369053, consult Aladdin Scientific technical support or the accompanying CoA.

Research Applications and Experimental Considerations

Recombinant Human LCE3C Protein is suitable for a range of in vitro research applications. Typical uses include:

• Protein interaction studies: Investigating binding partners of LCE3C within the cornified envelope scaffold using pull-down assays or surface plasmon resonance (SPR).
• Immunogen production: Generating antibodies against LCE3C for use in immunohistochemistry (IHC), Western blotting, or ELISA development.
• Functional assays: Studying the structural and biochemical role of LCE3C in epidermal barrier assembly.
• Disease model research: Supporting mechanistic studies in psoriasis and other inflammatory skin conditions linked to the PSORS4 locus deletion.
• Structural biology: Potential use in structural characterization studies of LCE family proteins.

Researchers should validate the protein for each specific application and confirm activity specifications with the datasheet or technical support prior to experimental use.

Ordering and Technical Support

Recombinant Human LCE3C Protein is available from Aladdin Scientific under SKU rp369053. To place an order or request additional product information:

• Visit the Aladdin Scientific website and search for SKU rp369053 or the product name.
• Contact your regional Aladdin Scientific sales representative for pricing, availability, and volume discount inquiries.
• For technical questions regarding experimental setup, assay compatibility, or protein activity, reach out to Aladdin Scientific technical support.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for the specific lot prior to use.

Aladdin Scientific is committed to providing high-quality recombinant proteins and responsive customer support to accelerate your research.

Frequently asked questions

What is Recombinant Human LCE3C Protein used for in research?

Recombinant Human LCE3C Protein is primarily used in studies of epidermal barrier function, keratinocyte terminal differentiation, and inflammatory skin diseases such as psoriasis. It is also used as an immunogen for antibody generation and in protein interaction assays to characterize the cornified cell envelope.

Why is LCE3C relevant to psoriasis research?

Deletion of the LCE3B and LCE3C genes at the PSORS4 locus on chromosome 1q21 has been genetically associated with increased susceptibility to psoriasis. Because LCE3C is a structural protein of the cornified envelope, its absence may compromise skin barrier integrity and predispose individuals to inflammatory responses. Recombinant LCE3C protein enables mechanistic investigation of this association.

How should I store Recombinant Human LCE3C Protein to maintain its stability?

For short-term use, store the protein at 4 °C. For long-term storage, aliquot the protein and keep it at −20 °C or −80 °C to prevent degradation from repeated freeze-thaw cycles. Always consult the product-specific Certificate of Analysis (CoA) for validated, lot-specific storage conditions.

What is the catalog number (SKU) for Recombinant Human LCE3C Protein at Aladdin Scientific?

Recombinant Human LCE3C Protein is available from Aladdin Scientific under SKU rp369053. You can search for this SKU directly on the Aladdin Scientific website or contact your regional sales representative to place an order.

Is the expression system and purity information available for SKU rp369053?

Detailed specifications including expression system, purity grade, and endotoxin levels are provided on the official product datasheet and Certificate of Analysis (CoA) for each lot. Researchers are encouraged to contact Aladdin Scientific technical support or download the CoA from the product page to obtain the most accurate and current specifications.

References

1. de Cid R, et al. Deletion of the PSORS4 gene LCE3B and LCE3C is associated with susceptibility to psoriasis. Nature Genetics, 2009.
2. Kypriotou M, Huber M, Hohl D. The human epidermal differentiation complex: cornified envelope precursors, S100 proteins and the 'fused genes' family. Experimental Dermatology, 2012.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.