Recombinant Human MAGED4 Protein: Key FAQs & Specs

Recombinant Human MAGED4 Protein is a research-grade recombinant protein derived from the human MAGED4 gene, offered by Aladdin Scientific (SKU: rp375988) to support studies in cancer biology, antigen characterization, and related biomedical research fields.

Product Name

Recombinant Human MAGED4 Protein

SKU

rp375988

Gene

MAGED4 (Melanoma Antigen Gene D4)

Protein Family

MAGE (Melanoma Antigen Gene) protein family

Species Origin

Human (Homo sapiens)

Application Area

Cancer biology, antigen research, immunology

Supplier Reference

absea#PP-9613

What Is MAGED4 and Why Is It Scientifically Relevant?

MAGED4 (Melanoma Antigen Gene D4) is a member of the MAGE (Melanoma Antigen Gene) protein family, a large group of proteins originally identified due to their aberrant expression in melanoma and other tumor types. MAGE family proteins are broadly classified as cancer-testis antigens (CTAs), meaning their expression under normal physiological conditions is predominantly restricted to male germ cells in the testis, while being re-expressed in a variety of malignant tumors.

MAGED4 belongs to the MAGE-D subfamily and has been implicated in cellular processes including apoptosis regulation, cell cycle control, and interactions with p75 neurotrophin receptor (p75NTR) signaling pathways. Its restricted expression profile in normal adult somatic tissues, combined with upregulation in certain cancers, makes MAGED4 an attractive subject for cancer biomarker research and immunotherapy target studies.

Recombinant Human MAGED4 Protein enables researchers to study protein function, generate or validate antibodies, and develop assay systems without relying on tumor-derived material.

Product Specifications and Quality Attributes

Recombinant Human MAGED4 Protein (SKU: rp375988) is produced as a recombinant protein intended for research use. The product is referenced under supplier code absea#PP-9613. Researchers should consult the official Aladdin Scientific product datasheet for confirmed details on the following attributes, as specifications may vary by lot:

• Expression System: Specific expression host (e.g., E. coli, insect, mammalian, or HEK293 cells) should be confirmed on the current product datasheet.
• Purity: Typical recombinant proteins from Aladdin Scientific are supplied at high purity (commonly >90% by SDS-PAGE); confirm exact purity on the datasheet.
• Tag Information: The recombinant protein may carry an affinity tag (e.g., His-tag) to facilitate purification; confirm tag identity and position from the datasheet.
• Formulation: The protein is typically supplied in a buffered solution; lyophilized formats may also be available. Confirm formulation and concentration from the datasheet.
• Molecular Weight: Confirm the expected molecular weight (including any tag) from the datasheet, as this will depend on the expressed construct.

Always refer to the lot-specific Certificate of Analysis (CoA) for quality control data including purity, activity, and endotoxin levels.

Storage, Handling, and Stability Recommendations

Proper storage and handling of Recombinant Human MAGED4 Protein are essential to maintain protein integrity, biological activity, and reproducibility of experimental results. The following guidance reflects general best practices for recombinant proteins; always defer to the product-specific datasheet for confirmed conditions:

• Short-term storage: Store at 4°C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: For longer storage periods, aliquot the protein to avoid repeated freeze-thaw cycles and store at −20°C or −80°C as indicated on the datasheet.
• Freeze-thaw cycles: Minimize freeze-thaw cycles, as repeated cycling can lead to protein aggregation, loss of activity, and degradation. Single-use aliquots are recommended.
• Carrier protein: If supplied at low concentrations, addition of a carrier protein (e.g., 0.1% BSA) may be recommended to prevent non-specific adsorption to tube surfaces; confirm with datasheet guidance.
• Avoid: Prolonged exposure to room temperature, repeated freeze-thaw, and contamination with proteases or detergents incompatible with the assay system.

If the protein arrives lyophilized, reconstitute according to the datasheet instructions using the recommended buffer and volume, allow to dissolve gently without vortexing, and allow equilibration before use.

Typical Research Applications

Recombinant Human MAGED4 Protein is intended for research use only and supports a range of in vitro and biochemical applications. Typical uses include, but may not be limited to:

• Antibody Production and Validation: As an immunogen for generating polyclonal or monoclonal antibodies against MAGED4, or as a positive control antigen in Western blot, ELISA, and immunoprecipitation assays.
• Protein-Protein Interaction Studies: Investigating binding partners of MAGED4 within MAGE-D subfamily signaling networks, including potential interactions with NRAGE, p75NTR, and apoptotic pathway components.
• Functional Assays: Cell-free biochemical assays examining MAGED4 enzymatic or regulatory activities, subject to confirmation of protein activity specifications on the datasheet.
• Biomarker Research: Development and optimization of immunoassays (ELISA, Luminex, etc.) intended to detect MAGED4 as a potential cancer biomarker in research samples.
• Structural Studies: Biophysical characterization including circular dichroism (CD), dynamic light scattering (DLS), or crystallography studies, subject to protein format and purity suitability.

This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use.

Ordering, Availability, and Customer Support

Recombinant Human MAGED4 Protein is available from Aladdin Scientific under SKU rp375988. Researchers and procurement teams can order directly through the Aladdin Scientific website or by contacting the Aladdin sales team.

For ordering inquiries, bulk quotations, or institutional purchasing arrangements, please contact Aladdin Scientific customer service. Custom protein services, including production of specific constructs or large-scale quantities, may also be available upon request.

When placing an order, please have the following information ready:

• Product SKU: rp375988
• Required quantity and format (e.g., liquid or lyophilized)
• Shipping destination and any cold-chain requirements
• Purchase order number or institutional billing information

For technical questions about this product—including application-specific guidance, troubleshooting, or datasheet clarification—please contact Aladdin Scientific Technical Support. Certificate of Analysis (CoA) documents for specific lots can be requested from customer service using the lot number printed on the product label.

Frequently asked questions

What is MAGED4 and what is the biological function of this protein?

MAGED4 (Melanoma Antigen Gene D4) is a member of the MAGE-D subfamily of cancer-testis antigens, proteins normally expressed in testicular germ cells but aberrantly re-expressed in various tumors. MAGED4 has been implicated in apoptosis regulation, cell cycle modulation, and interaction with the p75 neurotrophin receptor (p75NTR) signaling pathway. Its restricted normal-tissue expression profile and tumor upregulation make it relevant to cancer biomarker and immunotherapy research.

What expression system is used to produce Recombinant Human MAGED4 Protein (rp375988)?

The specific expression system used for Recombinant Human MAGED4 Protein (SKU: rp375988) should be confirmed by consulting the current product datasheet available on the Aladdin Scientific website or by contacting Technical Support. Common expression hosts for recombinant MAGE family proteins include E. coli, insect cells (baculovirus system), and mammalian cell lines such as HEK293. The datasheet will also specify tag information, molecular weight, and formulation details.

What are the recommended storage conditions for Recombinant Human MAGED4 Protein?

As a general guideline for recombinant proteins, Recombinant Human MAGED4 Protein should be stored at −20°C or −80°C for long-term preservation, with working aliquots stored at 4°C for short-term use (up to 1–2 weeks). Repeated freeze-thaw cycles should be avoided to prevent protein degradation and loss of activity. Always confirm the exact storage conditions, formulation, and reconstitution instructions from the lot-specific datasheet or Certificate of Analysis provided with your order.

For what research applications is Recombinant Human MAGED4 Protein validated?

Recombinant Human MAGED4 Protein is suitable for a range of research applications including antibody production, antibody validation (Western blot, ELISA, immunoprecipitation), protein-protein interaction studies, cancer biomarker assay development, and functional biochemical studies. Specific validated applications for SKU rp375988 are listed on the product datasheet; researchers should confirm compatibility with their intended assay format before use. This product is for research use only and is not intended for clinical or diagnostic applications.

How can I request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for this product?

Certificates of Analysis (CoA) for Recombinant Human MAGED4 Protein (rp375988) can be requested from Aladdin Scientific customer service by providing the lot number found on your product label. Safety Data Sheets (SDS) are typically available for download directly from the product page on the Aladdin Scientific website. For additional documentation or technical inquiries, contact Aladdin Scientific Technical Support with your order details.

References

1. Chomez P, De Backer O, Bertrand M, De Plaen E, Boon T, Lucas S. An overview of the MAGE gene family with the identification of all human members of the family. Cancer Res. 2001;61(14):5544-5551. PMID:11454705
2. Barker PA, Salehi A. The MAGE proteins: emerging roles in cell cycle progression, apoptosis, and neurogenetic disease. J Neurosci Res. 2002;67(6):705-712. PMID:11891778
3. Aladdin Scientific. Recombinant Human MAGED4 Protein (rp375988) Product Datasheet. Aladdin Scientific; [current version]. Available from: https://www.aladdin-e.com

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.