Recombinant Human MBLAC1 Protein: Properties & Applications
Recombinant Human MBLAC1 Protein: Properties & Applications
Recombinant Human MBLAC1 Protein is a research-grade recombinant enzyme belonging to the metallo-beta-lactamase superfamily, produced for use in biochemical assays, structural studies, and functional investigations of its conserved catalytic domain.
- Protein Name
- Recombinant Human MBLAC1 Protein
- Gene Name
- MBLAC1 (Metallo-Beta-Lactamase Domain-Containing Protein 1)
- Species
- Homo sapiens (Human)
- Protein Family
- Metallo-beta-lactamase superfamily
- SKU
- rp375205
- Supplier Reference
- absea#PP-8926
- Recommended Use
- Biochemical assays, protein-protein interaction studies, structural biology, enzyme activity research
Background and Biological Function of MBLAC1
MBLAC1 (Metallo-Beta-Lactamase Domain-Containing Protein 1) is a human protein that belongs to the large and functionally diverse metallo-beta-lactamase (MBL) superfamily. Members of this superfamily are defined by a conserved MBL fold and the ability to coordinate one or two metal ions, typically zinc, within their active site. While the superfamily is best known for bacterial enzymes that confer antibiotic resistance by hydrolyzing beta-lactam antibiotics, the human MBL-domain proteins serve distinct cellular roles unrelated to antibiotic resistance.
MBLAC1 is expressed in human tissues and has been studied in the context of nucleic acid metabolism and cellular signaling pathways. The conserved catalytic domain of MBLAC1 suggests a role in phosphodiester bond hydrolysis or related enzymatic activity, although the precise endogenous substrate and full physiological function of MBLAC1 in human cells continue to be areas of active investigation. Research has linked MBL-domain proteins to RNA processing, DNA repair surveillance, and metabolic regulation, making MBLAC1 of interest to researchers working across these disciplines.
Protein Specifications and Quality Standards
Recombinant Human MBLAC1 Protein (SKU: rp375205) is manufactured to research-grade standards. Recombinant proteins of this class are typically produced in a suitable heterologous expression system—such as Escherichia coli, insect cells (baculovirus), or mammalian cell lines—to yield soluble, functional protein. The choice of expression host influences post-translational modifications, folding, and biological activity.
Standard quality control measures applied to research-grade recombinant proteins include: SDS-PAGE analysis to confirm molecular weight and purity; Western blot verification using validated anti-MBLAC1 antibodies; endotoxin testing (LAL assay) to ensure suitability for cell-based assays; and functional activity assays where applicable. Researchers are advised to consult the product datasheet (absea#PP-8926) for the specific lot-dependent purity percentage, formulation buffer composition, recommended storage conditions, and validated activity data.
Note: Specific numerical purity values, host expression system details, and storage temperature are listed on the product-specific Certificate of Analysis (CoA) and datasheet. Please refer to the official Aladdin Scientific product page for the most current specifications.
Research Applications
Recombinant Human MBLAC1 Protein is suitable for a range of in vitro research applications. Researchers working in the following areas may find this protein particularly valuable:
- Enzyme Activity Assays: Investigating the substrate specificity and catalytic mechanism of the MBLAC1 MBL domain using synthetic or natural substrates.
- Protein–Protein Interaction Studies: Identifying binding partners of MBLAC1 through co-immunoprecipitation, pull-down assays, or surface plasmon resonance (SPR).
- Structural Biology: As a source of purified protein for X-ray crystallography, cryo-electron microscopy (cryo-EM), or NMR studies aimed at resolving the three-dimensional structure of the MBLAC1 domain.
- Antibody Validation: Serving as a positive control antigen for validating anti-MBLAC1 antibodies in Western blot, ELISA, or immunoprecipitation experiments.
- Drug Discovery and Screening: Supporting high-throughput screening campaigns to identify small-molecule modulators of MBLAC1 activity, particularly in the context of therapeutic target exploration within the MBL superfamily.
These applications reflect the broad utility of recombinant human proteins in both basic and translational research settings.
Handling, Storage, and Reconstitution Guidelines
Proper handling and storage of recombinant proteins are essential to maintaining their integrity and biological activity. The following general best-practice guidelines apply to Recombinant Human MBLAC1 Protein; always cross-reference with the specific product datasheet (absea#PP-8926) for lot-specific instructions:
- Storage: Recombinant proteins are generally stored at –80 °C for long-term stability. Avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity. Aliquot the protein upon first thaw.
- Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended in the datasheet (typically sterile PBS or a low-salt Tris-HCl buffer), adding carrier protein such as 0.1% BSA if required to stabilize dilute solutions.
- Working Conditions: Keep protein on ice during experimental setup. Use low-binding microcentrifuge tubes to minimize adsorption losses at low concentrations.
- Sterility: For cell-based assays, use aseptic technique and filter-sterilize (0.22 µm) the reconstituted protein where possible.
Disclaimer: These are general guidelines. Specific storage temperatures, buffer formulations, and stability data are provided on the product Certificate of Analysis and datasheet. Refer to the official Aladdin Scientific documentation for authoritative instructions.
Ordering Information
Recombinant Human MBLAC1 Protein is available from Aladdin Scientific. Use the details below when ordering or requesting a quote:
| SKU | Product Name | Supplier Reference |
|---|---|---|
| rp375205 | Recombinant Human MBLAC1 Protein | absea#PP-8926 |
For bulk quantities, custom formulations, or additional technical support, please contact the Aladdin Scientific technical sales team. Pricing and availability are subject to change; visit the official Aladdin Scientific website for the most current information.
Frequently asked questions
What is MBLAC1 and why is it scientifically significant?
MBLAC1 is a human protein containing a metallo-beta-lactamase (MBL) domain, placing it within a superfamily of metal-coordinating enzymes with diverse functions in nucleic acid metabolism, DNA repair, and cellular regulation. Its conserved catalytic architecture makes it a compelling target for biochemical characterization and potential therapeutic exploration.
What expression system is used to produce Recombinant Human MBLAC1 Protein (rp375205)?
The specific expression system for SKU rp375205 is detailed in the product datasheet (absea#PP-8926). Common systems for research-grade recombinant human proteins include E. coli, baculovirus-infected insect cells, and mammalian cell lines. Please consult the official datasheet or contact Aladdin Scientific technical support for lot-specific information.
How should Recombinant Human MBLAC1 Protein be stored to maintain activity?
As a general best practice for recombinant proteins, storage at –80 °C is recommended for long-term preservation. Repeated freeze–thaw cycles should be avoided by preparing single-use aliquots upon first thaw. Always refer to the product-specific Certificate of Analysis and datasheet for authoritative storage conditions.
Can Recombinant Human MBLAC1 Protein be used for antibody validation?
Yes. Purified recombinant MBLAC1 protein is well-suited as a positive control antigen for validating anti-MBLAC1 antibodies in Western blot, ELISA, and immunoprecipitation experiments, ensuring that the antibody specifically recognizes the target protein under the intended assay conditions.
Is Recombinant Human MBLAC1 Protein suitable for structural biology applications?
Recombinant human proteins produced at research grade are commonly used as starting material for structural biology techniques including X-ray crystallography, cryo-EM, and NMR spectroscopy. Suitability depends on the achieved purity, homogeneity, and folding state of the specific lot; please consult the datasheet and contact Aladdin Scientific for detailed purity and homogeneity data.
References
- Dominski Z. Nucleases of the metallo-beta-lactamase family and their role in DNA and RNA metabolism. Critical Reviews in Biochemistry and Molecular Biology. 2007;42(2):67-93. doi:10.1080/10409230701279118
- Daiyasu H, Osaka K, Ishino Y, Toh H. Expansion of the zinc metallo-hydrolase family of the beta-lactamase fold. FEBS Letters. 2001;503(1):1-6. doi:10.1016/S0014-5793(01)02686-2
- Aladdin Scientific. Recombinant Human MBLAC1 Protein (rp375205). Product Datasheet absea#PP-8926. Aladdin Scientific.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.