Sucrose water hemolysis test

Summary

The sucrose hemolysis test can be used (1) to observe whether hemolysis occurs by destruction of red blood cells, and (2) this test is often used in conjunction with the acid lysis test as a simple screening test for paroxysmal sleep hemoglobinuria.

Operation method

Sucrose water hemolysis test

Principle

At pH 7.4, human erythrocytes adsorb complement from the serum of homozygous normal subjects without hemolysis. PNH erythrocytes undergo hemolysis in PNH (paroxysmal, sleepy hemoglobinuria). Erythrocytes are susceptible to hemolysis in response to activated complement. Sucrose can cause hemolysis after activation of the classical route of complement through the C3 preactivator system.

Materials and Instruments

Anticoagulation
Sucrose solution
37℃ Incubator

Move

I. Experimental reagents: 92.4g/L sucrose solution.

II. Experimental operation: take fresh anticoagulated blood (citrate or oxalate anticoagulation) 0.5 ml and add it to 45 ml of sucrose solution, mix well and place it in 37℃ incubator for 30 min and then take it out for centrifugation, and the red color of the supernatant is positive (hemolysis) and the colorless supernatant is negative.

Caveat

1. The instrument used should be clean and dry to avoid hemolysis.

2. If sucrose is not available, glucose can be used instead.

Common Problems

Related Diseases:

Pediatric paroxysmal sleep hemoglobinuria, megaloblastic anemia.

This experiment was derived from Mudanjiang Medical College, Laboratory Guidance for Laboratory Specialties.

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