Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Immunoglobulins are the central effector molecules of adaptive humoral immunity. They are produced by B cells and their terminally differentiated plasma cells and contribute to pathogen clearance, maintenance of immune homeostasis, and the development of immunopathology through a dual ...
Isotype control antibody is a negative control reagent that has the same immunoglobulin class, subclass, and light chain type as the research antibody (experimental antibody) but does not bind to the target antigen.
Multiplexed immunohistochemistry (mIHC) is an in situ multiple labeling method based on tyramide signal amplification (TSA) technology, which can achieve simultaneous detection of multiple targets (usually 6-8 types) on a single tissue section. Its core principle is to covalently bind the target ...
Antibodies, also referred to as immunoglobulins (Ig), are significant Y-shaped glycoproteins produced by B-cells as a primary immune defense. These large proteins specially bind unique pathogen molecules that are known as antigens.
Techniques often explored alongside immunological experiments.
