Laboratory Protocols & SOPs Library

Tris-Buffered Saline Tween-20 (TBST)

Recipe

1.Dissolve the following in 800ml of distilled H2O

  • 8.8 g of NaCl

  • 0.2g of KCl

  • 3g of Tris base

2.Add 500ul of Tween-20

3.Adjust the pH to 7.4

4.Add distilled H2O to 1L

5.Sterilize by filtration or autoclaving

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Tris-Buffered Saline Tween-20 (TBST)" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/us_fr/faqs/tris-buffered-saline-tween-20-tbst-en.html
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