Frequently Asked Questions: Products, Experiments and Troubleshooting

What related reagents are needed for flow-through staining?

· Published 28 août 2024 · Updated 9 juin 2025

Samples with red blood cells, need to use red blood cell lysate, lysed red blood cells;
Fc receptor-rich cells such as macrophages need to be closed to FC receptor before adding the flow antibody to minimize non-specific binding of the antibody.
Cell staining buffer is required for the cell staining process;
Fixed rupture solution for intracellular indicator detection is required for intracellular indicator detection, and fixed rupture solution for intranuclear indicator detection is required for intranuclear indicator detection;
Dead cell dyes are also used for flow-through assays on tissue samples such as tumors.

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Categories: Frequently Asked Questions: Products, Experiments and Troubleshooting

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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Aladdin Scientific. "What related reagents are needed for flow-through staining?" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/us_fr/faqs/what-related-reagents-are-needed-for-flow-through-staining-en.html

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