GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human GAPDH Antibody, Monoclonal (B08/4A11), could be used for IF/ICC and Flow Cytometry.
Specifications
Product Name
GAPDH Mouse mAb (AF488) - Primary antibody, specific to GAPDH
Synonyms
38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Specificity
GAPDH
Immunogen
Recombinant full length human GAPDH
Positive Control
Flow Cytometry: Hela cells IF/ICC: MCF-7 cells
Conjugation
AF488
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images
GAPDH Mouse mAb (AF488) (Ab219255) – IF/ICC IF analysis of GAPDH (green) in MCF-7 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with GAPDH Mouse mAb (AF488) (Ab219255) at a dilution of 1/1000 in blocking buffer for 2 hour at RT. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
GAPDH Mouse mAb (AF488) (Ab219255) - Flow Cytometry Flow cytometry analysis of GAPDH expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (AF488) (Ab219255) (red) at 1/1000 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
IF/ICC
1/100-1/1000
Flow Cytometry
1:50-1:500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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