GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc
Host species
Mouse
Specificity
Human IL-6
Immunogen
Recombinant protein corresponding to Human IL-6
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Matched Pairs
[Ab220858] IL-6 Mouse mAb as Detection Antibody in ELISA
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Sandwich ELISA - IL-6 standard curve Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab220856) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab220858) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Detection range: 0.03 ng/mL - 2000.0 ng/mL
Indirect ELISA - IL-6 standard curve Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab220856). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody. EC₅₀: 29.72 ng/mL
Application
Application
Dilution info
ELISA
1.0 µg/mL as a Detection antibody in ELISA
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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