What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
Frequently Asked Questions: Products, Experiments and Troubleshooting
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
·Published ·Updated
What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?
The precipitation in the denatured protein buffer may be related to the ion concentration in the protein sample. If the precipitation occurs during the temperature change, it can be dissolved by incubation in a water bath at 37℃ or below. If there is still a small amount of precipitate that cannot be dissolved the supernatant can be centrifuged and used.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "What is the reason that the protein added to the buffer cooks without precipitation, but the next day when the sample is removed and spiked, precipitation appears? What should be done?" Aladdin Knowledge Base, updated 9 nov. 2026. https://www.aladdinsci.com/us_fr/faqs/the-protein-added-to-the-buffer-cooks-without-precipitation-en.html
You and 48 people found this helpful.
Nous utilisons des cookies pour garantir le bon fonctionnement du site et, lorsque cela est autorisé, pour améliorer votre expérience. Vous pouvez gérer vos préférences à tout moment dans les Paramètres. Pour en savoir plus, consultez notre Politique relative aux cookies.
Souhaitez-vous que nous vous envoyions un message lorsque des réductions seront disponibles?
Me rappeler plus tard
Merci ! Veuillez vérifier votre boîte de réception e-mail pour confirmer.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
