KP372-1 - 10mM in DMSO , CAS No.1374996-60-7

CAS: 1374996-60-7 Cat. No.: K655617 Molecular Weight: 448.4 EC Number: 808-876-3 PubChem CID: 49835991

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head and neck squamous cell carcinoma (HNSCC) and pancreatic cancer) .

In Vitro

KP372-1 (0.0625, 0.125, 0.25, 0.5, 1.0 µM; 48 h) inhibits growth of JMARc42 and Tu167c2 cells with IC 50 s of 200 and 100 nM, respectively. KP372-1 (125 nM; 24 h) induces Tu167c2 cells apoptosis and induces anoikis in the JMARc42 cells. KP372-1 (125 nM; 30 min) blocks Akt, thereby decreasing the phosphorylation of the S6 ribosomal protein in both Tu167 and JMAR cells. KP372-1 (0.250, 0.5, 1.0 µM; 30 min) inhibits Akt kinase activity with an IC 50 of 250 nM in JMAR cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: JMARc42 and Tu167c2 cells Concentration: 0.0625, 0.125, 0.25, 0.5, 1.0 µM Incubation Time: 48 h Result: Showed antiproliferative activity. Apoptosis AnalysisCell Line: Tu167c2 and JMARc42 cells Concentration: 125 nM Incubation Time: 24 h Result: Induced approximately 90% of cells apoptosis. Western Blot AnalysisCell Line: Tu167 and JMAR cells Concentration: 125 nM Incubation Time: 30 min Result: Induced a small but consistent decrease in Akt phosphorylation with a concomitant marked decrease in S6 phosphorylation. Inhibited the EGF induced phosphorylation of Aktser473 in Tu167 and AktThr308 in JMAR. Western Blot AnalysisCell Line: JMAR cells Concentration: 0.250, 0.5, 1.0 µM Incubation Time: 30 min Result: Significantly blocked Akt kinase activity in a dose-dependent fashion, with an IC 50 of 250 nM.

In Vivo

KP372-1(10, 20 mg/kg; i.v.; single daily for 33 days) induces NADH oxidation and impairs tumor growth in vivo without apparent toxicity. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Nude mice (H1299 xenografts model). Dosage: 10, 20 mg/kg Administration: Tailvein injection; single daily for 33 days Result: Affected tumor metabolism and suppressed tumor growth.

Specifications

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Isomeric SMILES: C1=CC=C2C(=C1)C3=NC4=NN=NN4N=C3C2=O.C1=CC=C2C(=C1)C3=NN4C(=NN=N4)N=C3C2=O
• WGK Germany: 3
• PubChem CID: 49835991
• Molecular Weight: 448.4