Mitochondrial complex I Activity Assay Kit (Micro Method)

Cat. No.: M1515822

GRADE & PURITY BioReagent

Vue d’ensemble

Mitochondrial respiratory chain complex I, also known as NADH-CoQ reductase or NADH dehydrogenase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is the largest protein complex in the inner mitochondrial membrane. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, while simultaneously reducing O₂ to generate O₂•⁻ (superoxide anion). It represents the primary step in the mitochondrial respiratory electron transport chain responsible for O₂•⁻ production. The activity level of this enzyme not only reflects the status of the respiratory electron transport chain but also indicates the state of reactive oxygen species generation. The detection principle of this kit is based on the ability of mitochondrial respiratory chain complex I to catalyze the dehydrogenation of NADH, generating NAD⁺. By measuring the change in absorbance of NADH at 340 nm, the activity of mitochondrial respiratory chain complex I in the sample can be calculated.

Specifications

Spécifications et pureté

BioReagent

Grade

BioReagent

Application

Enzyme activity assay

Type d'échantillon

Animal tissues, Plant tissues

Méthode de détection

Colorimetric Method

Detection instrument

Microplate reader

Detection wavelength

340nm

Stockage et expédition

Conditions de stockage de stockage

Protected from light,Store at -20°C

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at -20℃ for 6 months. Store in the dark.

Foire aux questions

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.