Mouse Coagulation Factor Ⅸ (FⅨ) ELISA Kit

Cat. No.: EJ1512678

QUALITÉ & PURETÉ BioReagent

Vue d’ensemble

This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse FⅨ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse FⅨ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.

Spécifications

Spécifications et pureté

BioReagent

Grade

BioReagent

Application

ELISA

Type d'échantillon

Plasma

Plage de détection

0.78ng/mL~50ng/mL

Espèces détectées Objet

Mouse

Spécificité

Detecting Mouse FⅨ in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Stabilité et stockage

Store at 2-8℃ for 6 months.