MTT Formazan - ≥97%(HPLC) , CAS No.23305-68-2

CAS: 23305-68-2 Cat. No.: M158055 Molecular Weight: 335.43 EC Number: 245-566-1

GRADE & PURITY ≥97%(HPLC)

Synonyms

A816662 | SCHEMBL745116 | MTT Formazan, powder | (E,E)-1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan | Thiazolyl blue formazan | SCHEMBL745118 | 2(3H)-Thiazolone, 4,5-dimethyl-,[phenyl(phenylazo)methylene]hydrazone | 1-(4,5-Dimethylthiazol-2-yl)-3,5-d

Storage

Store at 2-8°C,Protected from light,Argon charged

Shipped In

Wet ice

Overview

MTT Formazan has been used to measure cell viability.

Specifications

Synonyms

A816662 | SCHEMBL745116 | MTT Formazan, powder | (E, E)-1-(4, 5-dimethylthiazol-2-yl)-3, 5-diphenylformazan | Thiazolyl blue formazan | SCHEMBL745118 | 2(3H)-Thiazolone, 4, 5-dimethyl-, [phenyl(phenylazo)methylene]hydrazone | 1-(4, 5-Dimethylthiazol-2-yl)-3, 5-d

Specifications & Purity

≥97%(HPLC)

Biochemical and Physiological Mechanisms

The MTT method is suitable for detecting cell viability during apoptosis. Living cells reduce MTT (yellow) to MTT formazan (dark blue product). The amount of MTT formazan can be measured by spectrophotometry at 570nm.

Storage

Store at 2-8°C, Protected from light, Argon charged

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥97%(HPLC)

Names and Identifiers

• Pubchem Sid: 504768364
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504768364
• Canonical Smiles: CC1=C(SC(=N1)N=NC(=NNC2=CC=CC=C2)C3=CC=CC=C3)C
• IUPAC Name: N'-anilino-N-[(4,5-dimethyl-1,3-thiazol-2-yl)imino]benzenecarboximidamide
• InChIKey: GIFKVQOPAJDJLO-RHRKZKCTSA-N
• INCHI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22?
• Isomeric SMILES: CC1=C(SC(=N1)N=N/C(=N/NC2=CC=CC=C2)/C3=CC=CC=C3)C
• Alternate CAS: 57360-69-7
• Molecular Weight: 335.43
• Reaxy-Rn: 21761609
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=21761609&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Azoles
      • Subclass Thiazoles

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Azoles
• Subclass: Thiazoles
• Intermediate Tree Nodes: Not available
• Direct Parent: 2,4,5-trisubstituted thiazoles
• Alternative Parents: Phenylhydrazines Heteroaromatic compounds Formazans Azo compounds Propargyl-type 1,3-dipolar organic compounds Hydrazones Azacyclic compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Phenylhydrazine - 2,4,5-trisubstituted 1,3-thiazole - Benzenoid - Monocyclic benzene moiety - Heteroaromatic compound - Formazan - Azo compound - Azacycle - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Hydrazone - Organic nitrogen compound - Hydrocarbon derivative - Organonitrogen compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as 2,4,5-trisubstituted thiazoles. These are compounds containing a thiazole ring substituted at positions 2, 4 and 5 only.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: acetone: 1 mg/mL
• Sensitivity: Light & Air sensitive
• Melt Point(°C): 161-169℃
• Molecular Weight: 335.400 g/mol
• XLogP3: 5.900
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 5
• Exact Mass: 335.12 Da
• Monoisotopic Mass: 335.12 Da
• Topological Polar Surface Area: 90.200 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 441.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 1
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 1
• Covalently-Bonded Unit Count: 1

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