GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.PCR Reagent?PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.10×
Synonyms
PCR Reaction Buffer (10×, pH 8.3)
Storage
Store at -20°C,Avoid repeated freezing and thawing
BioReagent,PCR Reagent,sterile,10× BioReagent,PCR Reagent,Sterile for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
PCR Amplification Buffer (10×, pH 8.3) is a core auxiliary reagent for the Polymerase Chain Reaction (PCR). It provides a stable pH environment and necessary ionic conditions for the PCR reaction, ensures the activity of Taq DNA polymerase and other PCR-related enzymes, promotes the specific binding of primers to template DNA, and thereby facilitates the efficient amplification of target fragments.
The main components of this product include 100 mM Tris-HCl buffer (pH 8.3), 15 mM Mg²⁺, and 500 mM KCl, among others.
Procedure (For Reference Only)
Operate according to the specific requirements of the experiment.
PCR Amplification Buffer (10×, pH 8.3) should be used at a working concentration of 1×.
Notes
To avoid a decrease in amplification efficiency, repeated freezing and thawing should be avoided.
Prevent microbial contamination.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only and is strictly prohibited for other purposes.
Specifications
Synonyms
PCR Reaction Buffer (10×, pH 8.3)
Specifications & Purity
BioReagent, PCR Reagent, sterile, 10×
Stability And Storage
Store at -20℃ long term (12 months). Avoid freeze/thaw cycle.
Storage
Store at -20°C, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, PCR Reagent, Sterile
Names and Identifiers
PH
8.3
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.