PLSCR1 Human Pre-designed siRNA Set A

Cat. No.: P1479641

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

PLSCR1 Human Pre-designed siRNA Set A contains three designed siRNAs for PLSCR1 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components PLSCR1 siRNA-1: 5 nmol (HPLC) PLSCR1 siRNA-2: 5 nmol (HPLC) PLSCR1 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)

Specifications

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Product Line

Produits biologiques

Application Protocols

Only product-data–verified protocols can be listed here. For SKU P1479641, specific, tested application protocols, recommended dilutions, and validated cell lines/assays are Not specified for this item; refer to CoA/Spec Sheet.

Guidance

• Please consult the product’s CoA/Spec Sheet for any provided step-by-step transfection instructions, suggested working concentrations, and controls appropriate for PLSCR1 silencing. General, non-item-specific transfection guidance is provided in the Reaction Conditions tab.

Biological Roles

Target context (gene-level; literature, not item-specific)

• Gene: PLSCR1 (phospholipid scramblase 1), Homo sapiens.
• Canonical function: Facilitates Ca2+-dependent bidirectional translocation (“scrambling”) of phospholipids across the plasma membrane, dissipating lipid asymmetry under specific stimuli.
• Regulation: Interferon-inducible; transcriptionally upregulated in response to type I interferons and certain stress signals; subject to phosphorylation and palmitoylation that affect localization (literature).
• Cellular localization: Predominantly plasma membrane and endomembranes; also reported nuclear roles in transcriptional modulation (literature).
• Pathway connections: Links to antiviral innate immune signaling, apoptosis/anoikis, vesicular trafficking, and hematopoietic differentiation programs (literature).
• Phenotypic consequences of loss-of-function: Altered externalization of phosphatidylserine, changes in membrane curvature dynamics, and modulation of interferon-stimulated gene networks (cell-type dependent; literature).

Relevance for experiments

• Silencing PLSCR1 with siRNA enables dissection of lipid scrambling events, testing of calcium signaling dependencies, and evaluation of interferon-response cross-talk in human cell models.

Note

• The above roles summarize current literature and are provided for experimental planning. They are not item-specific performance claims. Sequence, modification status, and expected knockdown efficiency for this product are Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

Applicability

• As an siRNA reagent, this product is reconstituted and stored in aqueous, nuclease-free buffers. No classical “buffering” application (e.g., pH control in assays) is inherent to the siRNA itself.

General/literature guidance for siRNA handling buffers (not item-specific)

• Reconstitution: RNase-free water or TE buffer (10 mM Tris-HCl, 0.1–1 mM EDTA, pH 7.5–8.0). EDTA helps chelate trace divalent cations that can accelerate RNA cleavage.
• Working dilutions: Prepare in RNase-free water or serum-free transfection medium immediately before complex formation with a transfection reagent.
• Storage aliquots: Small, single-use aliquots minimize freeze–thaw cycles.
• pH range: 7.2–8.0 is commonly used to balance RNA stability with compatibility to cell culture (literature).
• Filtration: Avoid routine filtration through nucleic-acid–binding membranes; if sterilization is necessary, use low-binding PES and verify recovery (literature).

Item-specific details

• Exact buffer recommendations for this SKU are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Context

• For gene modulation in cultured cells, alternative modalities include plasmid overexpression, CRISPR knockouts/CRISPRi, and viral shRNA. Environmental impact considerations involve reagent toxicity, waste streams, and biosafety levels.

Comparative considerations (general/literature)

• Synthetic siRNA
  • Pros: No viral vectors; typically BSL-2 or lower; minimal persistent genetic alteration; aqueous handling; limited hazardous waste.
  • Cons: Transient effect; requires repeated dosing; reliance on cationic lipids (which may be cytotoxic) for delivery.
• Viral shRNA/CRISPR vectors
  • Pros: Stable knockdown/knockout; lower reagent consumption over time.
  • Cons: Viral packaging waste, antibiotics/selection waste, higher biosafety burden; plasmid prep solvents.
• Small-molecule inhibitors
  • Pros: Simple dosing; scalable.
  • Cons: Off-target pharmacology; organic solvent use; disposal considerations.

Greener practice tips

• Use serum-free complexing only for the minimal period, then restore serum to reduce cell stress and repeats.
• Choose low-toxicity, high-efficiency transfection reagents to reduce dose and waste.
• Consolidate experiments with pooled siRNA to minimize consumable use.
• Dispose of nucleic-acid–containing waste according to local guidelines; avoid bleach with cationic lipids to prevent noxious byproducts.

Pharmaceutical Uses

This product is supplied strictly for laboratory research use (from Product Data: Research Use Note — For research use only).

Not applicable for clinical/therapeutic use

• No pharmacopeial status, GMP grade, or clinical formulation details are provided. Any use as an active pharmaceutical ingredient, excipient, or in manufacturing of medicinal products is not indicated.

General note (non-clinical)

• In the research setting, siRNA tools are used for target validation and mechanism studies that can inform upstream discovery. This does not imply suitability for human or veterinary applications. For any regulated or translational work, consult appropriate GMP-grade materials and regulatory guidance.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature values for siRNA (not item-specific)

• Physical state: Dry solid (lyophilized) or aqueous solution; hygroscopic and highly water-soluble due to polyanionic phosphate backbone.
• Approximate size: A single 21-mer siRNA duplex typically has a molecular mass in the 13–14 kDa range, depending on sequence and any modifications (literature).
• Solubility: Freely soluble in nuclease-free water and low-salt buffers (e.g., 10 mM Tris, pH 7.5–8.0) (literature). High divalent cation concentrations can promote nuclease activity rather than affect solubility.
• pKa/logP/BP/MP/refractive index: Not applicable or not meaningful for large polyanionic biopolymers; typically not reported for siRNA (literature).
• UV absorbance: Strong at 260 nm; extinction coefficient depends on exact sequence; Not specified for this item; refer to CoA/Spec Sheet.

Practical notes

• Reconstitution: Typical working stocks are 10–100 µM in RNase-free water or TE buffer (literature). Exact instructions are Not specified for this item; refer to CoA/Spec Sheet.
• Concentration determination: A260 using sequence-specific ε values (if provided) or generic oligo approximations (literature).

Quality and Grades

Item-specific facts (from Product Data)

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpreting typical siRNA quality descriptors (general guidance)

• Purification level: siRNA may be supplied desalting-only, cartridge, PAGE, or HPLC purified. Higher purification reduces truncated species and improves knockdown consistency. The exact purification for this item is Not specified; refer to CoA/Spec Sheet.
• Duplexing/annealing: Some products ship as pre-annealed duplexes; others as single strands. This item is described as a pre-designed siRNA set; duplexing status is Not specified; refer to CoA/Spec Sheet.
• Sequence disclosure: Pre-designed sets often come with validated target regions and may include sequence IDs and design scores. For this item: Not specified; refer to CoA/Spec Sheet.
• RNase-free status and endotoxin: Critical for cell-based assays. Certification (RNase/DNase-free, endotoxin level) is Not specified; refer to CoA/Spec Sheet.
• QC testing: Typical tests include MALDI/ESI mass confirmation of each strand, HPLC traces, UV purity, and functional knockdown data in a reference cell line. For this item, specific QC metrics are Not specified; refer to CoA/Spec Sheet.

What “Set A” typically implies (general)

• Multiple non-overlapping siRNA designs against PLSCR1 to increase the chance of robust knockdown and enable pooling or deconvolution (literature). Exact number/composition: Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

Scope for this product type

• PLSCR1 Human Pre-designed siRNA Set A is intended for RNA interference (RNAi)-based, transient post-transcriptional gene silencing of PLSCR1 mRNA in human cells. Specific sequence details and validated applications are Not specified for this item; refer to CoA/Spec Sheet.

General/literature applications for siRNA (not item-specific)

• Gene function studies: Evaluate PLSCR1 roles in phospholipid scrambling, membrane asymmetry, vesicle trafficking, and interferon-inducible responses.
• Pathway mapping: Dissect signaling intersections of PLSCR1 with antiviral response elements, lipid metabolism, and apoptotic pathways.
• Phenotypic assays: Morphology, membrane phospholipid externalization (e.g., Annexin V binding as a readout), reporter assays, and lipidomics following knockdown.
• Control strategies: Use non-targeting siRNA, siGLO-type tracers, and positive control siRNA against a housekeeping gene to benchmark transfection efficiency (literature).
• Pooled vs. deconvoluted use: Pools can reduce off-target effects and increase knockdown probability; deconvolution identifies the most effective single duplex (literature).

Practical tips (literature)

• Optimize transfection reagent, cell density, and siRNA dose; typical working ranges: 1–50 nM final siRNA, 24–96 h assay windows depending on protein half-life.
• Validate silencing by RT-qPCR (24–48 h) and by immunoblot/functional assay (48–96 h).
• Minimize off-targets via low doses, chemically modified siRNA (if available), and rigorous controls. Exact modification status for this item is Not specified; refer to CoA/Spec Sheet.

Reaction Conditions

Scope

• There are no chemical “reactions” for this product in the synthetic sense. The relevant conditions pertain to cell transfection and gene silencing kinetics.

General/literature guidance for siRNA transfection (not item-specific)

• Cell density: Transfect at 40–70% confluency for adherent cells to balance viability and uptake.
• Dosing: Typical final siRNA concentration 1–50 nM; start with 10 nM and optimize downward to minimize off-target effects.
• Complex formation: Combine siRNA with a cationic lipid/polymer per reagent instructions; incubate 5–20 min at room temperature before adding to cells.
• Medium: Use serum-free medium for complexing; replace or supplement with serum-containing medium after 4–6 h if required by the transfection reagent.
• Incubation/assay windows: mRNA knockdown often detectable at 24–48 h; protein-level effects at 48–96 h, depending on protein half-life.
• Replicates/controls: Include non-targeting siRNA, mock-transfected, and positive control knockdown.
• Readouts: RT-qPCR for transcript levels; western blot/flow cytometry/activity assays for protein/function.

Item-specific notes

• Sequence, chemical modifications, recommended transfection reagents, and validated cell lines for SKU P1479641 are Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

Item-specific hazards (from Product Data)

• GHS classification: Not specified for this item; refer to SDS.
• Signal word: Not specified for this item; refer to SDS.
• H-statements/Pictograms: Not specified for this item; refer to SDS.

General guidance for siRNA oligonucleotides (literature/best practice)

• Expected hazards: siRNA is generally considered of low acute toxicity. Handle as a laboratory chemical/biological material; avoid inhalation of powders and contact with eyes/skin.
• RNase control: Use nuclease-free consumables and wear gloves to prevent RNase contamination and degradation of the product.
• PPE: Lab coat, nitrile gloves, and safety glasses. Use a dust mask or handle in a clean area if manipulating dry powders.
• Incompatibilities: Strong oxidants and nucleases (RNases). Avoid microbial contamination.
• First aid (overview): If inhaled, move to fresh air. For skin/eye contact, rinse with water. If ingested, rinse mouth with water. Seek medical attention as per institutional policy. Always defer to the SDS for authoritative instructions.
• Spill/cleanup: Avoid aerosolization; wipe with damp, RNase-inactivating solution; dispose per institutional guidelines.
• Waste: Dispose as nonhazardous laboratory biological/chemical waste unless local regulations require otherwise.

Storage and transport (from Product Data)

• Storage: Store at -20°C.
• Shipping: Shipped in ice chest with ice pads to maintain cold chain integrity.

Solvent Selection

Applicability

• As an siRNA oligonucleotide product, solvent considerations center on reconstitution and dilution rather than reaction media.

General/literature guidance (not item-specific)

• Primary solvent: RNase-free water for initial reconstitution. TE buffer (10 mM Tris-HCl, 0.1–1 mM EDTA, pH 7.5–8.0) is commonly used to enhance stability by chelating divalent cations.
• pH range: Near-neutral (pH 7–8) minimizes depurination and backbone cleavage.
• Ionic strength: Low-salt storage (≤50 mM NaCl) is typical; higher salt may be used transiently for annealing or formulation but can influence aggregation with polycations.
• Organic solvents: Avoid; alcohols (ethanol/isopropanol) can precipitate oligonucleotides and are used for cleanup, not storage.
• Compatibility with transfection: Final working solutions are usually prepared in serum-free transfection medium immediately before use; serum can be reintroduced after complex formation per transfection reagent instructions.

Selection notes

• Choose RNase-free water for short-term working stocks; TE for long-term frozen stocks.
• Avoid Mg2+/Ca2+ in storage buffers; include EDTA if long-term stability is required (literature).
• Filter sterilization is generally unnecessary if using nuclease-free components; avoid membrane materials that bind nucleic acids (e.g., nylon).

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage conditions: Store at -20°C.
• Shipping conditions: Shipped in an ice chest with ice pads.

Not specified for this item; refer to CoA/Spec Sheet

• Exact reconstitution solvent, target stock concentration, and annealing status
• Stabilizers, if any (e.g., RNase inhibitors, carrier RNA)
• Number and size of aliquots provided; container material

General handling reminders (for planning only; not item-specific)

• Minimize freeze–thaw cycles by preparing single-use aliquots upon first thaw.
• Thaw on ice; keep solutions cold during setup to reduce nuclease activity.
• Use certified RNase/DNase-free tubes, tips, and water.

Note

• Follow the specific instructions supplied with the item’s documentation if they differ from the generic guidance above.

Structure and Identity

Item-specific facts (from Product Data)

• SKU: P1479641
• Product name: PLSCR1 Human Pre-designed siRNA Set A
• Intended target: Human PLSCR1 (phospholipid scramblase 1) gene
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey / SMILES: Not applicable to complex oligonucleotide mixtures; not specified for this item.

General/literature description (not item-specific)

• Chemical class: Double-stranded small interfering RNA (siRNA) oligonucleotides.
• Typical architecture: 19–21 bp duplex with 2-nt 3′ overhangs (often dTdT) on each strand (literature).
• Functional groups: Repeating phosphate diesters, ribose (2′-OH) sugars, nucleobases (A, U, G, C). May include terminal phosphate or chemical modifications depending on design (not specified for this item).
• 2D/structural features: Two antiparallel RNA strands forming an A-form helix; anionic backbone with regularly spaced negative charges; base-paired interior with Watson–Crick pairing; overhangs (when present) enhance RISC loading (literature).

Notes

• "Pre-designed siRNA Set A" denotes a curated set of siRNA duplex(es) targeting non-overlapping regions of PLSCR1 mRNA; exact sequences, lengths, and any modifications are Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not typically applicable.

• As an siRNA oligonucleotide reagent, this product is not used as a synthetic building block or reagent in classical organic synthesis. It serves as a biological tool for gene silencing in cell-based systems.

For researchers seeking chemical synthesis applications

• Consider instead small-molecule phosphoramidites or protected nucleoside building blocks for oligonucleotide synthesis workflows.
• For biochemical assembly, enzymatic ligation or splint-based assembly of RNA fragments can be explored, but these are distinct from the intended use of this product.

Target Specificity

Item-specific information (from Product Data)

• Target: PLSCR1 (phospholipid scramblase 1), species: Human.

Not specified for this item; refer to CoA/Spec Sheet

• siRNA sequences and target exons/isoforms
• Off-target assessment data and in silico design scores
• Cross-reactivity with other scramblase family members (PLSCR2–5)
• Efficacy/knockdown percentage in validated cell lines
• Chemical modifications employed to enhance specificity (e.g., seed-region tweaks) if any

Foire aux questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.