RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Cat. No.: Ab124480

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

K92021_15B6

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human RALB Antibody, Monoclonal (K92021_15B6), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/2000
Protein Function:
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis.

Spécifications

Product Name

RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Synonymes

V ral simian leukemia viral oncogene homolog B antibody | GTP binding protein | RalB | RAS-like protein B | ras-related protein Ral-B | v-ral simian leukemia viral oncogene homolog B (ras related | GTP bindingprotein) | 5730472O18Rik antibody | dRalb anti

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Espèce hôte

Mouse

Spécificité

RALB

Immunogène

Recombinant human RALB (AA 31-200).

Contrôle positif

WB: Hep G2, HEK293, A431, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG2a

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - Western Blot
All lanes: RALB Mouse mAb (Ab124480) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 32.2 s

IHC: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - IHC
IHC analysis of human testis tissue by RALB Mouse mAb (Ab124480).
IHC was performed using sections of the formalin-fixed paraffin-embedded human testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RALB Mouse mAb (Ab124480) at 0.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in seminiferous ducts are positively stained at the cytoplasm membrane.

Application

Application

Dilution info

WB

1/1000

IHC

1/2000

Cibles associées (humaines)

RALB Tbio Ras-related protein Ral-B (0 Activities)

Discover Target: RALB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RALB Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.