RBC Lysis Buffer - 100 ml

Cat. No.: R665528

GRADE & PURITY 100 ml

Storage

Room temperature

Shipped In

Normal

Overview

This product uses the principle that the concentration difference of salt ions inside and outside cells can cause cell membrane swelling to lyse red blood cells. It is mainly used for the removal of red blood cells in experiments, such as the separation and purification of lymphocytes, the separation and purification of dispersed tissue cells through enzyme digestion, and the removal of red blood cells in experiments such as tissue cell egg white and nucleic acid extraction.

Component	R665528 100 ml
RBC Lysis Buffer	100 ml

Instructions for use:
1. Add 3 times the volume of red blood cell lysate to 1 volume of fresh whole blood (such as 1 ml of fresh whole blood and 3 ml of red blood cell lysate), gently vortex or invert and mix well.
Attention: If fresh tissue cells are processed, they need to be digested into a single cell suspension by trypsin or other enzymes, and then centrifuged to collect the cells before proceeding with subsequent operations.
2. Incubate on ice for 15 minutes, gently vortex and mix twice during this time. Note: After red blood cell lysis, the solution should be clear and transparent.
Collect white blood cells by centrifugation at 3.4 ℃ and 450 × g for 10 minutes, and carefully discard the supernatant.
4. Add twice the volume of red blood cell lysate to the above precipitate, gently vortex and resuspend white blood cells (if the initial blood volume is 1 ml, add 2 ml of red blood cell lysate).
Collect white blood cells by centrifugation at 5.4 ℃ and 450 × g for 10 minutes, carefully and thoroughly aspirate the supernatant.
6. Resuspend cells for subsequent experiments. Note: If extracting RNA, it is best to start using a solution without RNase from this step.

Specifications

Specifications & Purity

100 ml

Storage

Room temperature

Shipped In

Normal

References

1. Yuan Gao, Mengyao Mu, Yiju Wei, Bowen Yan, Hui Liu, Kai Guo, Mengmeng Zhang, Xiaohui Dai, Xiao Sun, David Tai Leong.  (2024)  Novel ultrathin ferrous sulfide nanosheets: Towards replacing black phosphorus in anticancer nanotheranostics.  Bioactive Materials,      [PMID:40115876] [10.1016/j.bioactmat.2024.09.035]