RIPA Buffer

Cat. No.: R488105

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.RIPA (Radio-Immunoprecipitation Assay) Buffer is supplied as a ready to use solution that requires no preparation. It minimizes non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur, enabling studies of relevant protein-protein interactions. It is widely used in applications such as immunoprecipitation since most antibodies and protein antigens are not adversely affected by the components of this buffer.RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies:Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD).Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells.RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™Affinity Gels.

Specifications

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL®CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0.', '

References

1. Yuan Juan, Pan Jianli, Zhang Xiaofang, Gao Rui.  (2024)  TRIM21 reduces H1N1-induced inflammation and apoptosis by regulating the TBK1–IRF3 signaling pathway in A549 cells.  ARCHIVES OF VIROLOGY,  169  (4): (1-10).  [PMID:38480558] [10.1007/s00705-024-05989-6]