RSL3 - 10mM in DMSO , CAS No.1219810-16-8

CAS: 1219810-16-8 Cat. No.: R407911 Molecular Weight: 440.88 PubChem CID: 1750826

GRADE & PURITY 10mM in DMSO

Synonyms

(1S,3R)-RSL3 | 1H-​Pyrido[3,​4-​b]​indole-​3-​carboxylic acid, 2-​(2-​chloroacetyl)​-​2,​3,​4,​9-​tetrahydro-​1-​[4-​(methoxycarbonyl)​phenyl]​-​, methyl ester, (1S,​3R)​-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

RSL3 RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
In vitro

Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, RSL3 inactivates GPX4 to induce ROS production from lipid peroxidation. RSL3\'s ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment.

In vivo

Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and erastin in mice.
Cell Data

cell lines：MOLT-4 cell lines and Reh (pre-B cells)

Concentrations：1 µg/ml

Incubation Time：16hrs

Powder Purity:≥99%

Specifications

Synonyms

(1S, 3R)-RSL3 | 1H-​Pyrido[3, ​4-​b]​indole-​3-​carboxylic acid, 2-​(2-​chloroacetyl)​-​2, ​3, ​4, ​9-​tetrahydro-​1-​[4-​(methoxycarbonyl)​phenyl]​-​, methyl ester, (1S, ​3R)​-

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

RSL3 ((1S, 3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner, exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Isomeric SMILES: COC(=O)[C@H]1CC2=C([C@@H](N1C(=O)CCl)C3=CC=C(C=C3)C(=O)OC)NC4=CC=CC=C24
• PubChem CID: 1750826
• Molecular Weight: 440.88

Associated Targets(Human)

GPX4 Tchem Phospholipid hydroperoxide glutathione peroxidase (1 Activities)

Discover Target: GPX4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

References

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13. Deshan Yao, Liuxiang Bao, Sichuan Wang, Meng Tan, Yuanyuan Xu, Tianxu Wu, Zhengang Zhang, Kaizheng Gong.  (2024)  Isoliquiritigenin alleviates myocardial ischemia-reperfusion injury by regulating the Nrf2/HO-1/SLC7A11/GPX4 axis in mice.  FREE RADICAL BIOLOGY AND MEDICINE,      [PMID:38734270] [10.1016/j.freeradbiomed.2024.05.012]

14. Huiqin Zhou, Yunjia Peng, Xinhua Huo, Bingqing Li, Huasheng Liu, Jian Wang, Gaihua Zhang.  (2025)  Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer’s Disease.  Journal of Inflammation Research,      [PMID:39959647] [10.2147/JIR.S497418]

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16. Jiajian Wang, Lanqing Zhang, Runfang Li, Rui Yang, Fan Yang, Fei Yu, Jihong Zhang.  (2025)  Gambogic acid restores wild-type-like p53 functions to induce ferroptosis in mutant p53 cancer cells.  BIOCHEMICAL PHARMACOLOGY,      [PMID:41213406] [10.1016/j.bcp.2025.117533]

17. Weichen Dong, Wenxin Zhang, Li Huang, Lulu Xiao, Yuanfei Luo, Linying Yuan, Yike Jiang, Anyu Liao, Hongting Zhao, Zhihui Liu, Jia Wang, Ying Zhao, Yulong Cai, Mengna Peng, Dong Yang, Yi Xie, Kuanyu Li, Wusheng Zhu.  (2026)  Restoring cardiolipin homeostasis mitigates cerebral ischemia–reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis.  Journal of Advanced Research,      [PMID:] [10.1016/j.jare.2026.03.014]