SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) - BioReagent, for PAGE, for western blot

Cat. No.: S767014

GRADE & PURITY BioReagent, for PAGE, for Western blot

Synonyms

enaturated PAGE protein loading buffer( Non reducing, 5X) | Denaturated PAGE protein loading buffer | Non-denatured Gel Sample Loading Buffer

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

SDS-PAGE, WB

Overview

SDS-PAGE Sample Loading Buffer (Non-reduced, 5X) is a 5-fold concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer does not contain reducing agents such as DTT/B-mercaptoethanol, which does not affect the disulfide bond in the chain and the disulfide bond between the chains of protein molecules. But the solution contains SDS type denaturing agents. Suitable for protein sample preparation and loading in denatured non reduced SDS-PAGE.
Precautions：
1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution;
2. This product is only used for scientific research and cannot be used for human experiments or treatments;
3. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
Instructions for Use：
1. Reagent preparation
1.1 Mix the protein tracer loading buffer (non reducing, 5x) with the protein sample in a ratio of 1:4;
2. Protein sample processing
2.1 Heat the protein sample in a boiling water bath for 3-5 minutes;
2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000rpm for 30 seconds:
3. Protein loading and electrophoresis
3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;
3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.

Specifications

Synonyms

enaturated PAGE protein loading buffer( Non reducing, 5X) | Denaturated PAGE protein loading buffer | Non-denatured Gel Sample Loading Buffer

Specifications & Purity

BioReagent, for PAGE, for western blot

Stability And Storage

Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.

Storage

Protected from light, Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for PAGE, for Western blot

Names and Identifiers

• PH: 6.2-7.2

Chemical and Physical Properties

• Sensitivity: Light sensitive

References

1. Tingting Liu, Yao Gu, AL-Ansi Waleed, Mingcong Fan, Li Wang, Yan Li, Haifeng Qian.  (2025)  Unveiling the relationship between heat-resistant structure characteristics and inhibitory activity in colored highland barley proteinaceous α-amylase inhibitors.  FOOD CHEMISTRY,      [PMID:39986068] [10.1016/j.foodchem.2025.143401]