TTC Sabouraud Medium - BioReagent

Cat. No.: T1528361

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For cultivation and enumeration of fungi

Specifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

Produits biologiques

Application Protocols

No manufacturer-validated protocols are provided for this specific item. Below are literature-based, general-purpose procedures (not product specifications). Adjust to your SOPs.

A. Plate preparation (literature/general):

• Dissolve the dehydrated medium in purified water per your target concentration. Autoclave at 121 °C for 15–20 min. Cool to ~50 °C.
• If using TTC separately, add a sterile-filtered TTC solution to the cooled sterile base with aseptic mixing. Pour plates and allow to solidify; dry surfaces before use.

B. Inoculation and incubation:

• Streak or spread inoculum onto plates. Incubate inverted at 25–30 °C for molds (up to 5–7 days) or 30–35 °C for yeasts (24–72 h), per organism requirements.

C. Interpretation:

• Look for red coloration within colonies indicating TTC reduction and metabolic activity. Record colony counts and morphology.

D. Controls:

• Include uninoculated sterility controls and positive-control strains known to reduce TTC.

E. Troubleshooting tips:

• Weak coloration: Increase TTC within literature-safe range or extend incubation.
• Inhibition: Lower TTC concentration or adjust incubation temperature/time.

Item-specific tested applications/dilutions: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

This section addresses the biological context of the medium and its indicator (literature/general).

• Fungal nutrition: Sabouraud formulations supply peptides, amino acids, and high carbohydrate content, supporting heterotrophic growth of yeasts and molds. The acidic pH (~5.6) reduces growth of many bacteria, favoring fungal recovery.
• Indicator mechanism: TTC (2,3,5‑triphenyl tetrazolium chloride) acts as an artificial electron acceptor in cellular respiration. Metabolically active cells reduce TTC to insoluble red formazan, depositing within colonies and enhancing visibility. The extent and rate of reduction depends on species, metabolic state, and TTC concentration.
• Selectivity nuances: Some fungi are sensitive to tetrazolium salts at higher concentrations, potentially reducing colony size or delaying growth. Optimization of TTC level is often required for delicate or slow-growing isolates.
• Application spaces: Environmental microbiology, food and beverage quality studies, and general mycology research commonly use Sabouraud media to culture Candida spp., Saccharomyces spp., and filamentous fungi (selection depends on method and supplements).
• Interactions with supplements: Addition of antibiotics (e.g., chloramphenicol) or antifungals (e.g., cycloheximide) tailors selectivity but may alter TTC reduction patterns (practice-dependent, not part of this item unless stated on the CoA).

Note: Performance varies by organism and protocol; include appropriate positive and negative controls when interpreting TTC-based visualization.

Buffer Applications

Not a buffering reagent per se. TTC Sabouraud Medium is a complete culture medium rather than a discrete buffer system.

Practical notes (literature/general):

• Working pH is typically preset by the formulation (around pH 5.6 for Sabouraud-type media). Minor adjustments may be made pre-sterilization if validated for your organisms.
• If a buffered environment is required (e.g., to stabilize pH during extended incubations), a separate buffer system (such as phosphate-citrate for acidic ranges) can be incorporated by method development, with careful validation to avoid growth inhibition.

For classical buffer recipes and pH ranges, consult dedicated buffering agents; this product is not intended as a standalone buffer.

Green Alternatives

While culture media are water-based and generally aligned with green chemistry principles (aqueous solvent, ambient-pressure processing), there are choices that can lower environmental footprint and improve safety.

Considerations (general):

• Aqueous preparation: Uses water as solvent—preferred under green chemistry guidelines.
• Energy use: Autoclaving is energy-intensive. Where validated and appropriate, consider alternative sterilization (e.g., validated membrane filtration for heat-labile components) or consolidated sterilization cycles to reduce energy consumption.
• Indicator selection: TTC is effective but can be toxic in concentrated form and produces persistent red formazan. Alternatives like neutral red or resazurin/resorufin systems may be suitable for specific applications; each has trade-offs in sensitivity and organism tolerance (literature comparison).
• Waste minimization: Prepare only the volume needed; solidify spent media and autoclave prior to disposal to reduce contamination risk and downstream treatment.

Illustrative comparison (literature; not product specifications):

• TTC Sabouraud vs. Sabouraud without indicator: Indicator-free media reduce chemical load but lose differential visualization.
• Sabouraud vs. Potato Dextrose: Both are aqueous and low-hazard; selection hinges on target organism performance rather than green metrics.

Operational green tips:

• Use high-efficiency autoclaves, load optimally, and employ reusable glassware where possible. Document cycle validation to avoid repeats that waste energy.

Pharmaceutical Uses

No therapeutic or clinical uses are claimed or implied. For research/lab and manufacturing support only.

Industry-relevant applications (literature/general):

• Quality studies in non-sterile product research: Sabouraud-based media are commonly used in laboratories to culture and study fungal contaminants from raw materials, utilities, and environmental samples.
• Environmental monitoring research: Air and surface sampling programs in research or pilot manufacturing spaces may utilize fungal cultivation on Sabouraud agar variants with or without TTC for colony differentiation.
• Microbial enumeration method development: Supports development of laboratory methods to enumerate yeasts/molds in complex matrices.

Standards and compendia:

• Pharmacopeial monographs exist for Sabouraud Dextrose Agar/Broth in certain contexts; formulations and acceptance criteria vary by compendium and are method-specific. This item’s exact compliance status is Not specified for this item; refer to CoA/Spec Sheet.

Note: Use is for research or process-support investigations only. Confirm suitability and any required compendial alignment in your internal methods.

Physical Properties

Because TTC Sabouraud Medium is a complex, dehydrated blend, single-compound physical constants do not apply. Item-specific physical values are not provided.

Item-specific values:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not applicable to a mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index, density, logP, pKa: Not applicable to a dehydrated mixture; Not specified for this item; refer to CoA/Spec Sheet.

Literature/general properties for Sabouraud-type media (for planning only; not product specifications):

• Typical preparation strength: 30–65 g/L depending on whether broth or agar is prepared; TTC is often used at 0.01–0.1 g/L, added aseptically (ranges from literature).
• Typical working pH: ~5.6 at 25 °C to favor fungi and suppress many bacteria (literature).
• Solubility: Freely soluble/dispersible in water with heating; forms a clear to slightly opalescent solution before sterilization (literature). If agar is present, a gel forms on cooling (literature).
• Thermal behavior: Designed to withstand autoclaving at 121 °C; TTC may be heat-labile in some contexts and is commonly filter-sterilized and added post-autoclave to maximize indicator performance (literature practice).

Practical note:

• Water quality (distilled/deionized), controlled heating, and thorough mixing are important to fully dissolve carbohydrates and peptones and to avoid localized caramelization of dextrose (general guidance).

Quality and Grades

• Grade/Purity: BioReagent (item-specific). This denotes suitability for laboratory biological applications, including preparation of culture media and general microbiology workflows. BioReagent grade emphasizes consistent performance and low levels of inhibitory contaminants relative to technical grades.

What BioReagent means in practice (general):

• Controlled composition of peptones/carbohydrates for reproducible growth performance.
• Low bioburden and low inhibitory residues to support target organism recovery.
• Batch-to-batch QC may include growth promotion testing (GPT) with representative yeast and mold strains and TTC color development (manufacturer-dependent; consult CoA for this item).

Stabilizers/additives:

• Not specified for this item; refer to CoA/Spec Sheet.

UV/metal/peroxide or other trace specifications:

• Not specified for this item; refer to CoA/Spec Sheet.

Documentation and verification:

• For regulated environments, retain lot-specific CoA and SDS. Where applicable, perform in-house verification (e.g., pH after sterilization, gel strength if agar-bearing, color/clarity, and TTC responsiveness) before critical studies.

Note: The product is listed for research use only; not intended for diagnostic or clinical use.

Reaction and Applications

Chemical reaction use is not applicable. Instead, TTC Sabouraud Medium is applied in microbiology workflows for cultivation and differentiation of fungi.

Applications (literature/general):

• Isolation and enumeration of yeasts and molds from environmental, food, and research samples. Acidic pH and elevated dextrose aid fungal recovery while suppressing many bacteria.
• Differential visualization with TTC: Actively respiring colonies reduce TTC to red, insoluble formazan, improving colony contrast on plates and facilitating enumeration and picking.
• Viability assessment: TTC reduction correlates with metabolic activity; can aid in distinguishing viable from non-viable colonies on dense plates.
• Colony morphology studies: Enhanced contrast highlights edges and surface features for strain comparison.

Practical tips (general):

• Pour plates at ~45–50 °C to minimize condensation; ensure homogeneous TTC distribution to avoid zonation of color intensity.
• For heat-sensitive additives, filter-sterilize and add after cooling the autoclaved base.
• Incubate plates inverted to reduce surface moisture; typical fungal incubation temperatures are 25–30 °C for molds and 30–35 °C for many yeasts (method-dependent).
• Monitor TTC concentration: Excess TTC can overly inhibit some organisms; too little yields weak color. Optimize within literature-reported ranges for your strains.

Quality controls:

• Include positive-control strains known to reduce TTC and negative controls to verify background.

Reaction Conditions

Classical reaction conditions do not apply. Instead, the relevant “conditions” are media preparation and sterilization parameters (literature/general guidance; not item specifications):

• Reconstitution strength: Follow your validated SOP or manufacturer CoA. Literature ranges: ~30–65 g/L for Sabouraud-type media depending on broth vs. agar; TTC often 0.01–0.1 g/L.
• Dissolution: Suspend powder in purified water with gentle heating and stirring until fully dissolved/dispersed. Avoid scorching sugars.
• pH: Adjust as needed before sterilization to approximately 5.6 at 25 °C for classical Sabouraud performance (if your method specifies).
• Sterilization: Autoclave base medium at 121 °C for 15–20 minutes. If using TTC separately, sterile-filter TTC (0.22 µm) and add aseptically to cooled (~45–50 °C) sterile medium to preserve indicator activity.
• Pouring plates: Dispense into sterile Petri dishes; allow to solidify (if agar-containing). Dry surface before use to prevent colony spreading.
• Incubation: Common ranges are 25–30 °C for molds (up to 5–7 days) and 30–35 °C for many yeasts (24–72 h), per method.

Quality checks:

• Verify final pH, absence of contamination (uninoculated control), and TTC responsiveness using a known reducer strain.

Safety and Handling

• GHS classification, Signal Word, H/P statements, and pictograms: Not specified for this item; refer to SDS.
• Primary hazards (general for dehydrated culture media with TTC; literature/precautionary):
  • Dust may cause mechanical irritation to eyes and respiratory tract; avoid inhalation and contact.
  • TTC is a tetrazolium salt; avoid ingestion and skin contact; handle solutions with care to prevent staining.
• Recommended PPE: Laboratory coat, safety glasses, and appropriate gloves (e.g., nitrile). Use a dust mask or handle in a fume hood if creating airborne particulates.
• Handling guidance:
  • Avoid moisture uptake; keep container tightly closed to prevent clumping and degradation.
  • Prepare media with clean glassware; avoid cross-contamination from biological materials.
• Incompatibilities (general): Strong oxidizers may degrade organic components; strong bases/acids may shift medium pH beyond intended range; avoid excessive heating that can caramelize sugars.
• First aid (overview; defer to SDS):
  • Inhalation: Move to fresh air; seek medical attention if irritation persists.
  • Skin contact: Wash with soap and water; remove contaminated clothing.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; seek medical advice if irritation continues.
  • Ingestion: Rinse mouth; seek medical attention.
• Waste: Autoclave spent plates/broths prior to disposal; dispose according to institutional biosafety and local regulations.

Solvent Selection

This product is a dehydrated culture medium intended for reconstitution in water; conventional solvent selection paradigms for organic synthesis do not apply.

Practical guidance (literature/general):

• Primary solvent: Purified water (distilled/deionized). Dissolve with moderate heating and stirring.
• Sterilization: Autoclave at 121 °C (15–20 min) for the base medium. To preserve TTC performance, many protocols filter-sterilize TTC separately (0.22 µm) and add aseptically after cooling the autoclaved medium to ~45–50 °C.
• Additive compatibility: Antibiotics (e.g., chloramphenicol, gentamicin), cycloheximide, or acid/base supplements are sometimes used in specialized fungal workflows; add by sterile filtration to cooled medium if included in your method (not part of this item unless stated on the CoA).

Comparison (general):

• vs. Sabouraud without TTC: TTC formulation enables visual differentiation of metabolically active colonies via red formazan deposition.
• vs. Potato Dextrose media: Sabouraud is more acidic and peptone-rich; often yields robust yeast growth and selective pressure against many bacteria.

Note: Water quality and container cleanliness materially affect performance; avoid high TOC or residual disinfectants that can inhibit growth or reduce TTC.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Room temperature (item-specific).
• Shipped In: Normal (item-specific).

Container handling:

• Keep tightly closed in a cool, dry place. Protect from moisture uptake and strong odors/contaminants.

Reconstitution (literature/general; not product specifications):

• Dissolve the dehydrated medium in purified water at the concentration required by your SOP or CoA. Heat with stirring to aid dissolution.
• Adjust pH if your method specifies (Sabouraud-type media are commonly set near pH 5.6 at 25 °C in literature).
• Sterilize by autoclaving at 121 °C for 15–20 minutes. If preserving TTC performance, add TTC by sterile filtration to the cooled sterile base (~45–50 °C) under aseptic conditions.
• For agar plates, pour into sterile Petri dishes and allow to solidify; store finished plates at 2–8 °C in sealed bags to limit dehydration and use within their validated shelf life (practice-dependent).

Stability:

• Unopened dehydrated medium is generally stable at room temperature until the labeled expiration date. Item-specific shelf life and any reconstituted-medium hold times are Not specified for this item; refer to CoA/Spec Sheet.

Research Use Note: For research use only.

Structure and Identity

TTC Sabouraud Medium is a complex, dehydrated microbiological culture medium formulated for the cultivation and differentiation of fungi (yeasts and molds) using a redox indicator.

• Item nature: Mixture/complex medium, not a single chemical compound; therefore no single molecular formula, SMILES, or InChIKey applies.
• Core components (literature/general):
  • Sabouraud base (peptones/enzymatic digests + high dextrose) designed for fungal growth at acidic pH.
  • TTC (2,3,5‑triphenyl tetrazolium chloride) as an electron-accepting indicator reduced by metabolically active cells to insoluble red formazan, enhancing colony contrast.
• Structural features (descriptive):
  • Heterogeneous blend of organic nutrients (peptides, amino acids, carbohydrates) and inorganic salts; may include agar in solid formulations (not specified for this item).
  • Indicator component (TTC) is an aromatic tetrazolium salt (literature), but its structural identifiers are not assigned to the overall medium.

Item-specific identifiers:

• CAS: Not applicable to a complex medium; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / Molecular weight: Not applicable to a mixture; Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey / SMILES: Not applicable; Not specified for this item; refer to CoA/Spec Sheet.

Notes:

• As a complex medium, batch composition conforms to internal manufacturing specifications rather than a single defined chemical structure.

Synthetic Utility

Not applicable to chemical synthesis. TTC Sabouraud Medium is not a building block, reagent, or solvent for organic reactions.

Useful laboratory utility (literature/general):

• As a prepared medium, it serves as a tool reagent for cultivating and visually differentiating fungi in microbiology workflows.
• The TTC indicator function can be leveraged in method development where metabolic activity readouts are needed (e.g., counting viable colonies, screening for respiratory activity among isolates).

For organic synthesis, consult appropriate reagents and solvents; this product’s value is strictly microbiological.

Target Specificity

Antigen/epitope target data do not apply. This product is not an antibody or affinity reagent.

Selectivity profile (literature/general):

• Organism preference: Designed to support fungi (yeasts and molds). The acidic pH and high dextrose content suppress many bacteria.
• Differential aspect: TTC enables visualization of metabolically active colonies via red formazan deposition; the intensity can vary by species and metabolic state.

Item-specific tested organisms, clone data, or species reactivity: Not specified for this item; refer to CoA/Spec Sheet.