18:0 mPEG2000 PE , CAS No.156543-00-9

CAS: 156543-00-9 Cat. No.: M1439958 分子式: (C₂H₄O)nC₄₃H₈₄NO₁₀P 分子量: 2000(Average)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

18:0 mPEG2000 PE (DSPE-mPEG2000) is a PEG-based phospholipid. 18:0 mPEG2000 PE can be used to synthesis liposomes for delivering cancer agents.

Specifications

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

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名前と識別子

分子量

2000(Average)

Application Protocols

The following general, literature‑based procedures illustrate common research uses. Adapt and optimize for your system; no item‑specific validated protocol is claimed.

• PEGylated liposome by thin‑film hydration:

  1. Dissolve matrix lipids (e.g., saturated PC and cholesterol) with 18:0 mPEG2000 PE (e.g., a few mol%) in chloroform/methanol.
  2. Evaporate to a thin film under N2/Ar; desiccate ≥2 h.
  3. Hydrate with prewarmed buffer while vortexing; perform 3–5 freeze–thaw cycles.
  4. Extrude through 200→100 nm membranes. Remove unencapsulated solutes by SEC.

• Post‑insertion onto preformed vesicles/nanoparticles:

  1. Warm both the dispersion and a concentrated stock of 18:0 mPEG2000 PE above the matrix transition temperature.
  2. Combine under gentle stirring for 10–60 min.
  3. Purify by SEC or ultrafiltration to remove unincorporated lipid.

• Micelle preparation:

  1. Dissolve lipid in ethanol or t‑butanol; add aqueous buffer slowly with stirring above the transition temperature.
  2. Dialyze or diafilter to remove organic solvent.

Characterization (recommended): DLS (size, PDI), zeta potential, SEC for free lipid removal, and, if needed, phosphorus assay or HPLC to quantify incorporation (literature/general).

Biological Roles

No intrinsic biological activity is claimed for this research reagent. The following describes general, literature‑based functional roles of PEGylated phosphatidylethanolamines in biomimetic systems:

• Membrane mimicry: The phospholipid anchor embeds into bilayers, allowing the PEG chain to extend into the aqueous phase and create a steric, highly hydrated brush that reduces protein adsorption and vesicle–vesicle interactions.
• Colloidal stabilization: The PEG corona increases the effective hydrodynamic radius and steric repulsion, lowering aggregation in saline and protein‑rich media during in vitro studies.
• Surface passivation: Incorporation into supported bilayers or liposomes used to coat nanoparticles/surfaces reduces nonspecific binding of biomacromolecules in analytical assays.
• Tuning interfacial properties: Chain length (C18:0) and PEG size (~2 kDa average) determine anchoring strength and brush thickness; saturated anchors afford stronger retention in gel‑phase lipids compared with unsaturated anchors (literature/general).

All uses are restricted to research and laboratory investigations. No medical or clinical claims are made for this product.

Buffer Applications

While 18:0 mPEG2000 PE is not a buffering agent, it is frequently hydrated and processed in common laboratory buffers during vesicle or nanoparticle preparation (literature/general):

• Typical hydration buffers: PBS, HEPES (10–20 mM, 150 mM NaCl), or citrate buffers near neutral pH to maintain lipid stability during thin‑film hydration.
• Additives: Low levels of chelators (e.g., EDTA) may be used to limit metal‑catalyzed oxidation of lipids; avoid strong detergents unless intentional mixed‑micelle formation is desired.
• Practical tips:
  • Warm buffers to above the lipid transition range to aid dispersion.
  • Filter buffers (0.22 µm) to reduce particulates prior to extrusion or microfluidic assembly.

For specific buffer compositions or pH tolerances required by your system, validate empirically; item‑specific compatibility data are not provided here.

Green Alternatives

Sustainability considerations here relate mainly to solvent choice and processing, since the active material is a specialty amphiphile.

• Greener solvent choices (literature/general):
  • Replace chlorinated solvents where feasible. Ethanol or isopropanol can dissolve PEG‑PE upon warming and are compatible with lyophilization or spray‑drying approaches.
  • t‑Butanol/water co‑solvent systems enable freeze‑drying of pre‑mixed lipid cakes without chlorinated solvents.
• Process intensification:
  • Use microfluidic mixing with ethanol as the solvent phase to assemble PEGylated liposomes/particles, reducing solvent volumes and energy input relative to rotary evaporation.
• Comparison (general):
  • Chloroform/methanol: excellent solvency, high hazard and environmental burden.
  • Ethanol (anhydrous): moderate solvency for PEG‑PE on warming, substantially greener; flammable but lower toxicity.
  • Isopropanol: similar to ethanol; sometimes better for PEG‑rich compositions.

Trade‑offs:

• Switching away from chloroform may require higher temperatures and longer dissolution times and can alter particle size distributions during assembly. Validate formulation quality (size, PDI, encapsulation) when altering solvent systems.

Pharmaceutical Uses

For research use only. No medical or clinical use is claimed. The following discusses general, literature‑based formulation roles of PEGylated PE lipids:

• Excipient role in research formulations: Functions as a steric stabilizer in liposomes and lipid‑based nanoparticles to improve colloidal stability and reduce nonspecific protein adsorption during in vitro studies.
• Processing compatibility: Commonly employed in ethanol‑injection, thin‑film hydration/extrusion, and microfluidic assembly workflows.
• Regulatory/compendial status: Not specified for this item; refer to CoA/Spec Sheet for any statements of compliance. Users operating under GMP should independently qualify the material and vendor.
• Interactions with other excipients (general): Typically blended with high‑Tm saturated phosphatidylcholines (e.g., DSPC) and cholesterol to balance membrane rigidity with surface stealth.

Any references to dosage, therapeutic endpoints, or clinical performance are outside the scope of this catalog entry and are not provided.

Physical Properties

Item‑specific numeric specifications are not provided in the Product Data; consult the CoA/SDS for definitive values. The following are general/literature characteristics of PEGylated phosphatidylethanolamines with mPEG2000:

• Physical state/appearance: Typically waxy solid to powder; hygroscopic tendencies possible (literature/general).
• Solubility (literature/general):
  • Good solubility in chloroform, dichloromethane, and mixtures of chloroform/methanol.
  • Dispersible in ethanol and isopropanol upon warming.
  • Forms micelles or incorporates into vesicles in aqueous buffers; solubilization often aided by heating above the lipid phase transition and gentle sonication.
• Density, refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Melting/phase transition behavior (literature/general): Saturated C18 PE segments exhibit high gel–liquid crystalline transition temperatures; PEGylation broadens/lowers cooperative transitions and imparts fluidity to the corona. Exact Tm for this item is not specified.
• pKa/logP: Not typically defined for amphiphilic polymers; headgroup is zwitterionic/neutralized and PEG is nonionic (literature/general).
• Critical micelle concentration (CMC): Often in the low µM range for DSPE‑PEG2000 in literature; exact CMC for this item is not specified.

Always verify any numeric requirement (e.g., solvent, temperature) with the CoA or by small‑scale trials, as PEG chain length distributions lead to polydisperse physical behavior.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• PEG polydispersity (general/literature): mPEG2000 refers to an average molecular mass of ~2 kDa; actual distributions are polydisperse. Reputable suppliers control dispersity and residual low‑MW PEG by GPC/SEC.
• Lipid identity & content: High‑quality PEG‑PE materials are typically characterized by 1H/13C NMR, 31P NMR, MALDI‑TOF/ESI‑MS (average MW), HPLC/UPLC purity, and residual solvents. Item‑specific methods/limits: Not specified for this item.
• Stabilizers/antioxidants: Not specified for this item; refer to CoA/Spec Sheet. In general practice, PEGylated lipids may be supplied without added stabilizers; users often add BHT/Tocopherol only if compatible with application.
• UV/fluorescence background: PEG‑PE lacks strong chromophores and is generally low‑UV absorbing above 210–220 nm (literature/general). Exact UV cutoff: Not specified.
• What this means for your experiments:
  • For analytical or formulation work (e.g., nanoparticle stealth layers), confirm lot‑specific MW distribution and lipid content when critical to performance (e.g., surface grafting density).
  • If working under GMP or regulated frameworks, obtain CoA/SDS and any pharmacopoeial compliance statements; none are claimed here.

Reaction and Applications

This material is primarily a formulation component rather than a stoichiometric reagent. Typical research applications (literature/general):

• Liposomes and vesicles:
  • Incorporation at low mol% into neutral or charged phospholipid bilayers to generate PEGylated (stealth) liposomes that resist protein adsorption and aggregation.
  • Enhances colloidal stability during extrusion, storage, and in complex media (e.g., serum‑containing buffers in in vitro studies).
• Micelles and mixed micelles:
  • Forms micelles or participates in mixed micelles with helper lipids/surfactants for solubilizing hydrophobic probes and APIs in formulation research.
• Nanoparticle surface modification:
  • Post‑insertion method: incubate pre‑formed liposomes or polymeric nanoparticles above the lipid transition temperature to insert the mPEG‑PE anchor into outer leaflets, imparting a PEG corona.
  • Co‑formulation: dissolve with other lipids (e.g., saturated PCs, cholesterol) in organic solvent, form a thin film, hydrate, and extrude to target size distributions.
• Supported lipid bilayers and biosensing:
  • Used to tune surface passivation and reduce nonspecific binding on sensor chips and vesicle fusion‑derived bilayers.

Practical tips:

• Work under inert gas during solvent evaporation to minimize oxidation of fatty chains.
• Control mol% of PEGylated lipid; excessive amounts can destabilize bilayers and reduce encapsulation efficiency.
• For post‑insertion, use temperatures above the main transition of the host bilayer lipid to facilitate insertion and lateral diffusion (literature/general).

Reaction Conditions

Conventional “reaction conditions” do not apply; however, assembly and processing conditions critically influence performance (literature/general):

• Thin‑film hydration and extrusion:
  • Dissolve lipids in chloroform or chloroform/methanol; dry under N2/Ar to a thin film; desiccate to remove residual solvent.
  • Hydrate with prewarmed buffer and agitate; perform multiple freeze–thaw cycles; extrude through polycarbonate membranes to the desired size distribution.
• Ethanol or isopropanol injection:
  • Prepare a warm ethanolic solution of the lipid mixture; inject into stirred, warm aqueous buffer; optionally dialyze to remove solvent.
• Post‑insertion:
  • Incubate preformed vesicles or nanoparticles with mPEG‑PE at temperatures above the host bilayer transition; remove free lipid by size‑exclusion or ultrafiltration.
• Temperatures and times: Use temperatures above the main phase transition of the host matrix lipid (e.g., high‑Tm saturated PCs) to facilitate mixing; exact values are system‑dependent and not specified for this item.
• Atmosphere: Work under inert gas during solvent evaporation and hydration to minimize oxidation.

Validate conditions on small scale and characterize assemblies by DLS (size/PDI), zeta potential, and, where applicable, cryo‑TEM.

Safety and Handling

• GHS classification, signal word, pictograms, H‑statements: Not specified for this item; refer to the SDS for authoritative hazard information.
• General safety (good laboratory practice):
  • Handle in a chemical fume hood to avoid inhalation of dust/aerosols.
  • Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves. Consider double‑gloving for extended solvent handling.
  • Avoid contact with strong oxidizers and strong acids/bases which may hydrolyze ester linkages or oxidize fatty chains (general guidance).
• Thermal/processing cautions:
  • Prolonged heating above the lipid’s transition range can promote hydrolysis/oxidation; use inert atmosphere (N2/Ar) when evaporating organic solvents from lipid films.
  • PEG segments can undergo thermo‑oxidative degradation; minimize exposure to elevated temperatures and air during film formation.
• Hygroscopicity and moisture:
  • PEGylated lipids can absorb moisture; keep containers tightly closed. Allow vials to equilibrate to room temperature in a desiccator before opening to prevent condensation.
• First‑aid overview (general):
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Ingestion/inhalation: Move to fresh air; rinse mouth; seek medical advice.
• Waste: Dispose according to institutional and local regulations for organic chemical waste; avoid release to the environment.

Always defer to the SDS supplied with the lot for specific hazards, spill response, and transport information.

Solvent Selection

• Polarity and amphiphilicity: 18:0 mPEG2000 PE is amphiphilic—hydrophobic C18 chains and a hydrophilic, nonionic PEG headgroup—enabling dissolution in organic solvents and dispersion/self‑assembly in water (literature/general).
• Organic solvents (preferred for stock solutions and thin‑film formation):
  • Excellent: chloroform, dichloromethane, chloroform/methanol (2:1 to 9:1 v/v)
  • Good upon warming: ethanol, isopropanol, t‑butanol
  • Less suitable: highly nonpolar alkanes (limited solubility due to PEG block)
• Aqueous media:
  • Disperses to form micelles or incorporates into liposomes; hydration buffers commonly include PBS, HEPES, or citrate buffers at pH 6.5–7.5 (literature/general).
  • Gentle heating above the lipid phase transition and/or brief bath sonication aids dispersion.
• When to choose this amphiphile vs alternatives:
  • Choose mPEG2000‑PE when a robust steric corona is desired on vesicles, liposomes, or nanoparticle surfaces to reduce nonspecific adsorption and aggregation.
  • Consider shorter PEG (e.g., mPEG1000‑PE) for higher packing density with thinner coronas; consider longer PEG (e.g., mPEG5000‑PE) for thicker hydration layers, at the expense of membrane packing and Tm.

Small comparison (literature/general):

• mPEG‑PE vs PEGylated cholesterol: mPEG‑PE anchors via two acyl chains for higher retention in bilayers; PEG‑cholesterol offers different membrane fluidity and partitioning.
• mPEG‑PE vs Pluronic block copolymers: mPEG‑PE integrates into bilayers; Pluronics form micelles and adsorb to surfaces but are not lipid‑anchored.

Storage and Reconstitution

• Storage conditions (item‑specific): Store at -20°C.
• Shipping (item‑specific): Shipped in an ice chest with ice pads.
• Handling:
  • Allow the sealed vial to warm to room temperature in a desiccator before opening to prevent condensation on hygroscopic material.
  • Reseal promptly; purge headspace with dry nitrogen/argon if possible.
• Light and oxygen: Protect from light and air to limit oxidation of lipid chains; store under inert atmosphere if long term.
• Reconstitution (general/literature):
  • Organic stocks: Prepare ~5–20 mg/mL solutions in chloroform or chloroform/methanol. Store aliquots at -20°C, protected from moisture and air.
  • Aqueous dispersions: Hydrate prewarmed buffer with the dry film or inject ethanolic solution into buffer above the transition temperature; brief bath sonication can aid clarity.
• Freeze–thaw: Avoid repeated freeze–thaw of aqueous dispersions; store as small aliquots at 2–8°C for short term. For longer storage, keep as dry film or organic stock at -20°C.
• Stability: Item‑specific shelf life and per‑lot assay limits are not specified; refer to CoA/Spec Sheet and monitor by DLS/HPLC or 31P NMR where critical.

For research use only.

Structure and Identity

Amphiphilic PEGylated phospholipid commonly known as a methoxy‑PEG2000 conjugate of a saturated C18:0 phosphatidylethanolamine (PE).

• Product name: 18:0 mPEG2000 PE
• CAS: 156543-00-9
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (Note: PEG2000 denotes an average PEG chain mass of ~2000 Da; overall MW is polydisperse in literature.)
• SMILES/InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general/literature description):
  • A glycerophospholipid backbone bearing a phosphoethanolamine headgroup.
  • The ethanolamine nitrogen is derivatized via an amide or carbamate linker to a methoxy‑terminated poly(ethylene glycol) chain of average 2000 Da (mPEG2000).
  • One saturated C18:0 (stearoyl) acyl chain is explicitly specified; the second acyl chain is typically saturated C18 in analogous materials in literature. Exact acyl composition for this item: Not specified; refer to CoA/Spec Sheet.
  • Amphiphilic block‑copolymer‑like architecture: hydrophobic lipid tails + hydrophilic PEG corona.
• 2D structure in words: glycerol sn‑1/sn‑2 positions esterified with long C18:0 chains; sn‑3 phosphate linked to ethanolamine; the ethanolamine nitrogen bears a pendant PEG chain capped with methoxy. No stereochemical claims are made for this item unless specified in the CoA.

Synthetic Utility

This product is not commonly used as a reagent for small‑molecule synthesis. Its value lies in supramolecular and materials assembly (literature/general):

• Functional anchoring block: The dual C18:0 lipid anchor provides strong bilayer insertion, while the PEG chain presents a hydrophilic brush for surface modification of colloids and interfaces.
• Modular platform: mPEG‑PE can be co‑formulated with functional lipids (e.g., maleimide‑PEG‑PE, biotin‑PEG‑PE, or charged lipids) to introduce reactive handles or recognition motifs on vesicle or nanoparticle surfaces.
• Surface engineering: Facilitates the creation of PEGylated supported lipid bilayers on silica or polymer substrates for biosensing and antifouling studies.
• Retrosynthetic perspective: Not a building block for stepwise organic synthesis; rather, it is a macromolecular excipient for bottom‑up assembly of nanostructures.

If downstream chemical modification of the PEG terminus is required, consider using functionalized analogs (e.g., NHS‑PEG‑PE, maleimide‑PEG‑PE). This specific product is described as methoxy‑terminated; item‑specific functionality beyond mPEG is not specified.

Target Specificity

Not applicable. 18:0 mPEG2000 PE is a PEGylated lipid excipient, not a biological targeting reagent or antibody. No antigen/epitope specificity, clone, isotype, or species reactivity applies to this product.

よくある質問

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 156543-00-9, the molecular formula is (C2H4O)nC43H84NO10P, and the molecular weight is 2000(Average) g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.