2X Laemmli Sample Buffer - 2X , CAS No.X378399

CAS: X378399 Cat. No.: X378399

GRADE & PURITY 2X

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
2X Laemmli Sample Bufferis for SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer补充资料This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.

Specifications

仕様と純度

2X

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

名前と識別子

WGKドイツ

3

UN番号

3082

包装グループ

III

化学的性質と物理的性質

• 溶解度: Soluble in water.pH'~6.8