3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

Cat. No.: T755511

GRADE & PURITY peroxidase substrate

概要

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H₂SO₄, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Specifications

Product Name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

仕様と純度

peroxidase substrate

分子タイプ

生物試薬／緩衝液

保管と配送

集中

peroxidase substrate

保管条件

Store at 2-8°C

入荷

Wet ice

参考文献

1. Yingxue Li, Tingrui Zhang, Gang Bai, Mengchun Chen, Xiaodong Lei, Li Ye, Hua Yu, Zhichao Fan, Tao Yu.  (2024)  A target-triggered colorimetric sensor for ultrasensitive detection of miRNAs based on self-powered three-dimensional DNA walker.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:39265909] [10.1016/j.ijbiomac.2024.135370]

よくある質問

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.