GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Rabbit anti Human ACOT12 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/200 IF/ICC: 1/50-1/200 Protein Function Hydrolyzes acetyl-CoA to acetate and CoA.
Specifications
Product Name
ACOT12 Antibody - 一次抗体, specific to ACOT12, Rabbit IgG
A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACOT12.
ポジティブコントロール
WB: Mouse liver, Rat liver, Hep G2, HEK293 and A549 lysates. IHC: Human liver cancer tissue. IF/ICC: A549 cells.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Rabbit IgG
SDS-PAGE
150 kDa
精製方法
Immunogen affinity purified
集中
1.0 mg/mL
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
ACOT12 Antibody (Ab087205) - Western Blot All lanes: ACOT12 Antibody (Ab087205) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 57 kDa Exposure time: 200.0 s
ACOT12 Antibody (Ab087205) - Western Blot All lanes: ACOT12 Antibody (Ab087205) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 57 kDa Exposure time: 69.3 s
ACOT12 Antibody (Ab087205) - IHC Immunohistochemical analysis of ACOT12 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ACOT12 Antibody (Ab087205) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ACOT12 Antibody (Ab087205) - IF/ICC Immunofluorescent analysis of ACOT12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ACOT12 Antibody (Ab087205) in 3% BSA-PBS and incubated overnight at 4 °C in a humified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
適用＃テキヨウ＃
適用＃テキヨウ＃
Dilution info
IF/ICC
1/50 - 1/200
IHC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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