Actin-Tracker Red-555

Cat. No.: A743602

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

概要

This product should be aliquoted properly to avoid repeated freeze-thaws.For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .Phalloidin is toxic (LD50: 2mg/kg body weight). Less than 0.2mg of phalloidin is contained in 1ml of Actin-Tracker Red. Please take effective protections when using it.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Fluorescent staining of fixed cells or tissue sectionsa.Wash the cells or tissue sections twice with PBS.b.Fix the cells or tissue sections at room temperature for about 10 minutes with 's Immunol Staining Fix Solution or 3.7% formaldehyde solution prepared with PBS. Note: Fix solutions containing methanol should not be used, because methanol can destroy actin proteins. c.Wash samples 2-4 times with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100 for about 5 minutes each time.d.Dilute the Actin-Tracker Red-555 1:40-1:200 with 's Immunol Fluorescence Staining Secondary Antibody Dilution Buffer or PBS containing 1-5% BSA and 0.1% Triton X-100 (e.g., dilute 5μl or 25μl of Actin-Tracker Red-555 in 1ml of dilution buffer), to obtain the Actin-Tracker Red-555 working solution. Note: The dilution ratio can be adjusted based on the staining result.e.Add 200μl of Actin-Tracker Red-555 working solution to each slide and incubate at room temperature for 20-60 minutes in the dark. To prevent evaporation, it is better to place the slide in a Staining Box for Microscope Slides (, FSR958).f.Wash the slides 2-4 times with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100 for about 5 minutes each time.g.Examine the stained samples directly by fluorescence microscopy. For long-term storage, the slide can be mounted after air-dry and stored at 4℃ in the dark for up to 6 months.2.Fluorescent staining of live cells：Phalloidin is usually not cell-permeable and is therefore rarely used for live cell staining. However, it has been reported that live cells can be labeled by phalloidin which possibly enters live cells by phagocytosis. In general, a larger amount of fluorescence-conjugated phalloidin is needed for staining live cells.

仕様

安定性と保管

Store at -20 ℃ away from light, effective for 1 year.

保管条件

Protected from light,Store at -20°C

入荷

Ice chest + Ice pads

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Product Line

生物製品