AIF Mouse mAb - 一次抗体, specific to AIFM1, Mouse IgG2a

Cat. No.: Ab087780

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, WB

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG2a

Clone Number

8H1-B10-A12

Conjugation

Unconjugated

概要

Mouse anti Human AIF Antibody, Monoclonal(8H1-B10-A12), could be used for WB, IF, ICC and so on .
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Specifications

Product Name

AIF Mouse mAb - 一次抗体, specific to AIFM1, Mouse IgG2a

同義語

AIF | AIFapoptosis-inducing factor 1, mitochondrial | AIFM1 | apoptosis-inducing factor, mitochondrion-associated, 1 | COXPD6 | MGC111425 | PDCD8 | PDCD8striatal apoptosis-inducing factor | programmed cell death 8 (apoptosis-inducing factor) | Programmed

仕様と純度

ExactAb™, Validated, 1.0 mg/mL

宿主種

マウス

特異性

AIFM1

免疫原

Recombinant protein of human AIF (AA 1-170)

ポジティブコントロール

WB: HeLa, Ramos, HepG2, MCF-7, Jurkat lysates. ICC/IF: HeLa cells.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Mouse IgG2a

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

1.0 mg/mL

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: AIF Mouse mAb (Ab087780)

AIF Mouse mAb (Ab087780) - Western Blot
All lanes: AIF Mouse mAb (Ab087780) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 24.0 s

IF/ICC: AIF Mouse mAb (Ab087780)

AIF Mouse mAb (Ab087780) - IF/ICC
Immunocytochemistry analysis of AIF in HeLa using AIF Mouse mAb (Ab087780).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

関連ターゲット（人間）

AIFM1 Tbio Apoptosis-inducing factor 1, mitochondrial (0 Activities)

Discover Target: AIFM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (AIF Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.