Alkyne tyramide - 10mM in DMSO , CAS No.1694495-59-4

CAS: 1694495-59-4 Cat. No.: A655029 分子式: C₁₃H₁₅NO₂ 分子量: 217.26 PubChem CID: 108021005

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

概要

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also facilitates the identification of APEX-labeling sites, allowing the unambiguous assignment of membrane topology of mitochondrial proteins.

In Vitro

Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome. ?\nAPEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM. ?\nAlkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Specifications

仕様と純度

10mM in DMSO

生化学的・生理学的メカニズム

Alkyne tyramide is a clickable ascorbate peroxidase 2 (APEX2) probe. Alkyne tyramide substantially improves APEX-labeling efficiency in intact yeast cells, as it is more cell wall-permeant than APEX2 substrate biotin-phenol (BP). Alkyne tyramide also faci

保管条件

Store at -80°C

入荷

Dry ice packs + Cold packs

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名前と識別子

• 異性体SMILES: C#CCCC(=O)NCCC1=CC=C(C=C1)O
• PubChem CID: 108021005
• 分子量: 217.26