≥95% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Description
APN-Azide is a bifunctional crosslinker for thiol-to-alkyne coupling. This coupling can be performed with high selectivity in biological medium using mild reaction conditions.Standard protein labeling procedure (cysteine labeling)1. Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.2. Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).3. Incubate at room temperature for 2 hours.4. If necessary, purify the protein-azide conjugate using size exclusion chromatography or ultrafiltration.5. The conjugate can be readily coupled with alkyne-containing using standard click-chemistry protocols.*Note: avoid thiol-containing buffers.
| カノニカル・スマイル | N#CC#CC1=CC=C(N=[N+]=[N-])C=C1 |
|---|---|
| InChIKey | BDLXITYEYYHQNK-UHFFFAOYSA-N |
| INCHI | 1S/C9H4N4/c10-7-1-2-8-3-5-9(6-4-8)12-13-11/h3-6H |
| UN番号 | 1325 |
| 分子量 | 168.15 |
| Reaxy-Rn | 27679920 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=27679920&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| 感度 | light sensitive |
|---|---|
| 引火点（°F） | Not applicable |
| 引火点（°C） | Not applicable |
| 融点（°C） | 100℃ |
L2510163