AUT1 - 10mM in DMSO , CAS No.1311136-84-1

CAS: 1311136-84-1 Cat. No.: A655619 分子式: C₁₈H₁₉N₃O₄ 分子量: 341.36

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

概要

AUT1 is a recombinant human Kv3 channel modulator. AUT1 modulates Kv3.1b and Kv3.2a channels in human recombinant with pEC 50 values of 5.33 and 5.31 μM, respectively. AUT1 can be used for the research of disorders associated with dysfunction of inhibitory feedback in corticolimbic circuits, such as schizophrenia

In Vitro

AUT1 (1.5, 12.5, and 25 μM) modulates Kv3.1b and Kv3.2a channels in human recombinant with pEC 50 s of 5.33 and 5.31 μM, respectively. AUT1 increases whole currents mediated by human Kv3.1b and Kv3.2a channels in a concentration-dependent manner. AUT1 (10 and 30 μM) shifts both the voltage dependence of activation and inactivation of human Kv3.1b and Kv3.2a channels. AUT1 rescues (1 and 10 μM) the fast firing of EGFP-positive cortical interneurons. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:pEC50: 5.33 μM(Kv3.1b),5.31 μM (Kv3.2a)

Specifications

仕様と純度

10mM in DMSO

生化学的・生理学的メカニズム

AUT1 is a recombinant human Kv3 channel modulator. AUT1 modulates Kv3.1b and Kv3.2a channels in human recombinant with pEC 50 values of 5.33 and 5.31 μM, respectively. AUT1 can be used for the research of disorders associated with dysfunction of inhibitor

保管条件

Store at -80°C

入荷

Dry ice packs + Cold packs

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

アクションタイプ

MODULATOR

名前と識別子

• 分子量: 341.36

Application Protocols

No vendor-validated protocols are provided for this item. The following is general guidance for small-molecule screening and is not item-specific.

Example: Cell-based dose–response assay (general)

• Prepare a 10 mM stock in DMSO; confirm clarity. Make serial 1:3 dilutions across 10 points.
• Seed cells in 96- or 384-well plates and allow to attach/equilibrate per cell-line SOP.
• Dose to achieve a final DMSO ≤0.5% v/v. Include vehicle, positive, and negative controls.
• Incubate for an assay-appropriate period (e.g., 4–48 h).
• Read out via luminescence/fluorescence/imaging per assay design. Analyze with a 4PL fit to obtain EC50/IC50.

Example: Biochemical enzyme assay (general)

• Prepare assay buffer (e.g., HEPES, pH 7.5, with required cofactors and 0.01% Tween-20).
• Pre-incubate enzyme with compound dilutions; initiate with substrate.
• Monitor initial rates; include counter-screens to assess fluorescence quenching/activation or redox interference.

These examples are templates; adapt to your specific system. For item-specific protocols, refer to the CoA/Spec Sheet if provided.

Biological Roles

• Item-specific biological function, targets, or pathways for AUT1 are not specified in this listing. Any biological role would be dependent on disclosed structure and validated activity data.

General considerations for small-molecule tool compounds

• Library members like AUT1 are used to perturb biological systems to generate mechanistic hypotheses. Confirmed roles may include enzyme inhibition/activation, receptor modulation, or effects on signaling, trafficking, or metabolism, but such roles must be established empirically.
• If activity is observed, apply orthogonal assays and counter-screens to rule out frequent hitters (e.g., redox cyclers, PAINS, aggregators).
• For putative target identification, consider thermal shift assays (CETSA/TPP), chemoproteomics (affinity capture, photo-crosslinking if suitable analogs are available), or genetic interaction mapping.
• ADME-relevant profiling (permeability, efflux liability, microsomal stability) can contextualize cellular readouts, but those data are not provided for this item.

Note: No medical or clinical use is claimed or implied. AUT1 is provided strictly for research use only as stated in the product data.

Buffer Applications

AUT1 is a small-molecule screening compound and is not a buffering agent. There are no item-specific buffer recipes associated with this product.

Practical assay buffer guidance (general)

• Prepare assay buffers compatible with your biology (e.g., HEPES, PBS, Tris), keeping final DMSO ≤0.1–1% v/v when dosing from DMSO stocks.
• Include detergent (e.g., 0.01–0.05% Tween-20) for aggregation-prone readouts, if consistent with assay biology.
• Maintain consistent pH and ionic strength to avoid confounding solubility/ionization changes. Filter (0.22 µm) and degas if needed for optical or microfluidic assays.
• For enzymology, incorporate cofactors and divalent cations as required; verify that AUT1 does not chelate or interfere with detection chemistry.

If unique buffer conditions for AUT1 are later disclosed in the CoA/Spec Sheet, defer to those item-specific instructions.

Green Alternatives

Because AUT1 is supplied as a research screening compound rather than a process solvent or reagent, “greener alternative” discussion focuses on dissolution and assay vehicle choices.

• Prefer ethanol or water-buffer systems when solubility and assay biology allow, to reduce reliance on high-toxicity aprotic solvents.
• Use DMSO sparingly in assays (≤0.5% v/v preferred) and standardize vehicle controls to limit solvent burden.
• For analytical sample prep, prioritize MeOH over ACN where compatible with detection, given MeOH’s generally lower environmental impact.

Illustrative comparison (general, not item-specific)

• DMSO: high solvency; moderate EHS profile; widely accepted in screening.
• EtOH: renewable routes available; biological impact at higher %.
• MeOH: greener than ACN; toxic if ingested; compatible with many LC–MS methods.
• ACN: excellent chromatography solvent; supply chain and toxicity considerations.

Operational greening

• Reduce volumes via miniaturized assays (384/1536-well) to cut solvent use.
• Implement sealed plates to limit evaporation and VOC emissions.
• Centralize solvent waste segregation and distillation/recovery where permitted.

Given the lack of item-specific solubility/structure data, select the least hazardous solvent that achieves reliable, artifact-free performance in your assay.

Pharmaceutical Uses

No pharmacopeial status or excipient role is provided for this item.

• Formulation role: Not specified for this item; refer to CoA/Spec Sheet.
• Regulatory status: Research use only; not for human or veterinary use, diagnostic, or therapeutic applications.

General development context (for research planning only)

• If AUT1 shows promising research activity, early preformulation screens (solubility vs. pH, co-solvents, amorphous dispersions) and stability studies (thermal, oxidative, photolysis) can inform follow-up experiments.
• For in vitro use, qualify limits of vehicle tolerance (DMSO/EtOH) and adsorption to plastics. For in vivo exploratory research (outside the scope of this listing), comprehensive safety and regulatory evaluation would be required.

No clinical, therapeutic, or diagnostic claims are made for this product.

Physical Properties

Item-specific physicochemical data are not provided in the current listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point (BP): Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility profile: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General/literature-based guidance for screening compounds (not item-specific)

• Many discovery compounds are supplied as solids suitable for preparing DMSO stocks (e.g., 10–50 mM). If aqueous work is needed, co-solvent strategies (DMSO → buffer dilution) or cosolvent systems (EtOH/water, ACN/water) are typical.
• If the structure becomes available, compute pKa and cLogP to predict ionization and permeability; adjust pH to improve solubility when weakly acidic/basic.
• Perform a quick solubility check: weigh ~1 mg, test dissolution in 100 µL each of DMSO, DMF, MeOH, EtOH, ACN, PBS (pH 7.4), and record clarity after vortex/sonication.
• Assess stability by short-term incubation (e.g., 24–48 h) at assay temperature in the intended matrix; analyze by LC–MS for degradation, aggregation, or adsorption.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret grade for screening compounds (general guidance)

• Discovery compounds are commonly supplied at ≥95% purity (HPLC/LC–MS); however, actual thresholds vary by supplier and by intended use (HTS vs. in vivo). Because grade is not specified here, confirm via CoA before critical experiments.
• If present, a stabilizer or counterion will be listed on the CoA. Stabilizers can impact UV background and biological assays; consider a quick blank test if high-sensitivity detection is used.
• Analytical identity is typically supported by LC–MS and/or 1H NMR. Request spectra if your quality system requires vendor traceability.
• For HTS, low UV absorbance impurities and minimal residual solvents are preferred. If UV cutoff or residual solvent limits are critical, verify on the CoA/Spec Sheet (not provided in the current listing).
• If plate-ready format is required (e.g., pre-dissolved in DMSO at a defined molarity), inquire about custom services or prepare internally using calibrated balances and volumetrics.

Reaction and Applications

As listed, AUT1 is part of a small-molecule/compound library and is primarily intended as a screening tool compound rather than as a synthetic reagent.

Typical research applications for library compounds (general guidance)

• Primary/secondary screening: Evaluate activity in biochemical or cell-based assays; use concentration–response curves (e.g., 10-point, 1:3 dilutions) to estimate potency.
• Phenotypic assays: Measure cellular readouts (viability, reporter activity, imaging-based features) to generate hypotheses for mechanism of action.
• Target deconvolution: If activity is detected, pursue chemoproteomics, CETSA/TPP, CRISPR modifier screens, or panel profiling.
• SAR exploration: Use analog-by-catalog searches keyed to the CAS to source close analogs for rapid structure–activity mapping.
• Orthogonal confirmation: Reproduce hits across independent assay modalities and counter-screens to exclude artifacts (fluorescence interference, redox cycling, aggregation).

Practical tips

• Prepare fresh DMSO stocks and minimize freeze–thaw to retain assay consistency.
• Include vehicle controls matching the highest solvent concentration and positive/negative controls per assay best practices.
• Assess colloidal aggregation risk (e.g., detergent add-ins, light scattering), especially above 10–20 µM.

No manufacturer-specific reaction chemistry (e.g., named reactions) is applicable because the compound is not positioned as a building block or reagent in this listing.

Reaction Conditions

No item-specific reaction conditions are applicable because AUT1 is not offered as a reagent and its structure is not disclosed here.

General handling/usage conditions for screening compounds

• Stock preparation: Commonly 10–20 mM in anhydrous DMSO; sterile-filter (0.22 µm PTFE) if sterility is required for cell culture assays and solubility permits.
• Assay dosing: Titrate to achieve desired final concentrations while keeping vehicle constant (e.g., ≤0.5% DMSO). Use multi-point concentration–response (at least 8–10 points) for potency estimation.
• Stability checks: Incubate working solutions under assay conditions and assess by LC–MS for degradation over typical assay durations (1–48 h).
• Light/oxygen sensitivity: When unknown, protect from light and minimize headspace oxygen; consider nitrogen backfill.

If literature describing AUT1 (by CAS 1311136-84-1) reports specific conditions or targets, adopt those as appropriate—ensuring consistency with your assay system and institutional safety requirements.

Safety and Handling

Hazard classification for this specific item is not provided in the listing. Handle as a research chemical of unknown toxicity.

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word / H-Statements / Pictograms: Not specified for this item; refer to SDS.
• Primary storage guidance: Store at −80°C (per Product Data). Minimize freeze–thaw cycles by aliquoting.
• Shipping: Shipped on dry ice packs + cold packs (per Product Data). Unpack promptly and restore to −80°C.

General laboratory precautions (not item-specific)

• Use in a chemical fume hood with appropriate PPE: lab coat, safety glasses, and nitrile gloves. Avoid inhalation, ingestion, and skin/eye contact.
• Prevent moisture ingress and photodegradation when unknown: work under dry air or nitrogen if feasible; use amber vials/foil wrap.
• Incompatibilities are unknown; keep separate from strong oxidizers/acids/bases until compatibility is confirmed.
• First aid (overview; defer to SDS): rinse eyes/skin with water for ≥15 minutes upon contact; move to fresh air if inhaled; seek medical attention after any exposure.
• Waste: collect in an organic waste stream unless aqueous and nonhazardous is confirmed; follow institutional hazardous waste procedures.

Always consult the product’s SDS for authoritative safety information once available.

Solvent Selection

Item-specific solvent compatibility is not provided. The guidance below outlines a robust approach for small-molecule screening compounds like AUT1.

• Preferred stock solvent (general): DMSO due to broad solvating power and assay compatibility at ≤0.1–1% v/v final.
• Secondary options: DMF (strong solvency; higher toxicity), EtOH/MeOH (greener, protic; may affect cells/enzymes), ACN (volatile, good for LC compatibility), buffered aqueous media when ionization permits.
• Practical workflow:
  • Start with 10–20 mM in anhydrous DMSO. If insoluble, test DMF, then co-solvent blends (e.g., DMSO:water 1:1 for ionizable compounds).
  • For biological assays, maintain final co-solvent ≤0.5% where feasible; include vehicle controls.
  • For analytical work, ACN/water or MeOH/water with 0.1% formic acid often improves LC–MS behavior.

General comparison (not item-specific)

• DMSO: highest success rate; hygroscopic; can oxidize over time.
• EtOH: greener; limited for highly lipophilic compounds; affects membrane integrity at >1–2%.
• DMF: powerful solvent; handle with caution; harder to remove.
• ACN: volatile; good for chromatography; moderate solvency.

Because AUT1’s structure is not disclosed here, verify solubility empirically before committing to screening campaigns.

Storage and Reconstitution

• Storage (item-specific): Store at −80°C (per Product Data). Keep tightly closed. Protect from moisture and light.
• Shipping (item-specific): Dry ice packs + cold packs (per Product Data). Restore to −80°C upon receipt.

Reconstitution and handling (general guidance; not item-specific)

• Allow vials to equilibrate to room temperature in a desiccated environment before opening to avoid condensation.
• Dissolve in anhydrous DMSO to prepare a convenient stock concentration (e.g., 10–50 mM), adjusting based on observed solubility.
• If aqueous solutions are required, dilute DMSO stocks into buffer with vigorous mixing; maintain final DMSO at the lowest effective level (commonly ≤0.5% v/v). Consider adding a small amount of nonionic detergent to mitigate aggregation if compatible with the assay.
• Aliquot stocks into single-use portions in inert, low-bind tubes. Avoid repeated freeze–thaw cycles.
• For long-term storage, keep aliquots at −80°C; for short-term working stocks, −20°C may be acceptable if stability is confirmed experimentally.
• Inspect solutions for precipitation, discoloration, or haze before use; discard if instability is observed.

Item-specific stabilizers, counter-ions, or solubility data are not specified in this listing; consult the CoA/Spec Sheet for definitive instructions.

Structure and Identity

Brief overview: AUT1 (SKU A655619) is offered as a research-use small molecule from Aladdin Scientific’s screening compound collection.

• Product Name: AUT1
• SKU: A655619
• CAS: 1311136-84-1
• Category: Small molecules and compound libraries (screening compound)
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features

• Item-specific structural details (functional groups, ring systems, stereochemistry) are not provided in the current listing. For definitive structural assignment and cheminformatics usage (e.g., similarity search, docking), please consult the product’s CoA/Spec Sheet or request the structure disclosure if available under your screening agreement.

2D/cheminformatics description (general guidance)

• In the absence of a structure, reference the CAS in databases to retrieve any disclosed topology, calculate physicochemical descriptors (logP, polar surface area, HBD/HBA), and guide solvent selection and assay design.
• For inventory and ELN traceability, log SKU, CAS, batch/lot, and any internally assigned structure ID if known.

Synthetic Utility

AUT1 is supplied as a screening compound for biological/chemical biology studies and is not positioned as a synthetic reagent or building block in this listing.

• Without a disclosed structure, no specific functional-group reactivity, protecting-group strategies, or named reaction participation can be described for this item.
• If your program requires derivatization for SAR/target ID (e.g., biotinylation, photoaffinity, or clickable tags), consult structure disclosure (if available) or consider requesting analog sourcing based on the CAS to access similar scaffolds amenable to modification.
• For analytical derivatization (e.g., to improve ionization or detection), method development should be guided by known functionality—none is provided here.

In summary, consider AUT1 primarily as a tool compound; leverage close analogs or custom synthesis routes once the structure and IP constraints are clarified.

Target Specificity

Target, pathway, and binding data are not specified for this item.

• No antigen/epitope, enzyme, receptor, or pathway specificity is provided in the product data.
• If AUT1 is used as a tool compound in your lab, document any hypothesized targets and validate via orthogonal methods (kinetics, CETSA/TPP, pull-downs, genetics).

For authoritative target information, consult the CoA/Spec Sheet or primary literature associated with the CAS 1311136-84-1, if available to you.

よくある質問

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1311136-84-1, the molecular formula is C18H19N3O4, and the molecular weight is 341.36 g/mol.