グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.0.6 mg/mL
Rabbit anti Human BDH2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase.
仕様
Product Name
BDH2 Antibody - 一次抗体, specific to BDH2, Rabbit IgG
同義語
3-hydroxybutyrate dehydrogenase type 2 antibody | BDH 2 antibody | BDH2 antibody | BDH2_HUMAN antibody | Dehydrogenase/reductase (SDR family) member 6 antibody | Dehydrogenase/reductase SDR family member 6 antibody | DHRS6antibody | EFA6Rantibody | 3 hydr
仕様と純度
ExactAb™, Validated, Carrier Free, 0.6 mg/mL
宿主種
ウサギ
特異性
BDH2
免疫原
Fusion protein of human BDH2 (AA 1-245)
ポジティブコントロール
WB: HeLa, Jurkat, SK-N-SH and Hep G2 cell lysates. IHC: Human liver cancer tissue. IF/ICC: HeLa cells.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Rabbit IgG
SDS-PAGE
150 kDa
精製方法
Antigen affinity purified
集中
0.6 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
BDH2 Antibody (Ab090899) - Western Blot All lanes: BDH2 Antibody (Ab090899) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 26 kDa Exposure time: 0.1 s
BDH2 Antibody (Ab090899) - IHC Immunohistochemistry analysis of paraffin-embedded human liver cancer tissue labelling BDH2 with BDH2 Antibody (Ab090899) at 1/100 dilution. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
BDH2 Antibody (Ab090899) - IF/ICC
IF analysis of BDH2 Antibody (Ab090899) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with BDH2 Antibody (Ab090899) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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