CD133 Mouse mAb - 一次抗体, specific to Human CD133

Cat. No.: Ab169234

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

AB01/2H3

Conjugation

Unconjugated

概要

Mouse anti Human CD133 Antibody, clone AB01/2H3, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

CD133 Mouse mAb - 一次抗体, specific to Human CD133

仕様と純度

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

生化学的・生理学的メカニズム

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal

宿主種

マウス

特異性

Human CD133

免疫原

Recombinant protein corresponding to Human CD133

ポジティブコントロール

Flow Cytometry: HeLa cells

活用

Unconjugated

等級

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

ライトチェーン・タイプ

kappa

SDS-PAGE

150.0 KDa

精製方法

Protein A purified

集中

1.0 mg/mL

保管と配送

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

画像

Flow Cytometry: CD133 Mouse mAb (Ab169234)

CD133 Mouse mAb (Ab169234) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with CD133 Mouse mAb (Ab169234) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L(Ab213557) was used as an isotype control (Blue).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• Flow Cytometry:

  1/800 – 1/50000;

関連ターゲット（人間）

PROM1 Tbio Prominin-1 (0 Activities)

Discover Target: PROM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD133 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.