CD25 Rat mAb (AF647) - 一次抗体, specific to CD25

Cat. No.: Ab211659
注文可能
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:650nm ? Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:668nm ? Fluorescence emission maximum at 668 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
助言
Reactive species
マウス
Isotype
Rat IgG1
Clone Number
PC-61.5.3
Conjugation
AF647
Size
USA
ドイツ (EU)*
Price
Qty
10μg
Ab211659-10μg
4 在庫あり
$71.90
25μg
Ab211659-25μg
4 在庫あり
$141.90
100μg
Ab211659-100μg
2 在庫あり
$355.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL Em:668nm,Ex:650nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

概要

Rat anti Mouse CD25 Antibody, Monoclonal (PC-61.5.3), could be used for Flow Cytometry

Specifications

Product Name
CD25 Rat mAb (AF647) - 一次抗体, specific to CD25
同義語
Anti-mouse CD25 (IL-2Rα)-InVivo | In Vivo MAb anti-mouse CD25 (IL-2Rα) | CD25 Rat mAb | CD25 | Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor sub
仕様と純度
ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
宿主種
助言
特異性
CD25
免疫原
IL-2-dependent cytolytic mouse T cell clone B6.1
ポジティブコントロール
Flow: C57BL/6 mouse splenocytes
活用
AF647
励起波長(nm)
Red Laser(633nm)
発光波長(nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
等級
Em:668nm, Ex:650nm, ExactAb™, Validated
ライトチェーン・タイプ
lambda
精製方法
Protein A purified
集中
0.5 mg/mL
保管と配送
保管条件
Store at 2-8°C,Protected from light,Do not freeze
入荷
Wet ice,Do not freeze
安定性と保管
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
画像

CD25 Rat mAb (AF647) (Ab211659) - Flow Cytometry
Con A-stimulated (2 days) C57BL/6 mouse splenocytes (right panel) stained with CD25 Rat mAb (AF647) (Ab211659) (red) compared to an isotype control (blue). Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

適用#テキヨウ#
適用#テキヨウ#Dilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

関連ターゲット(人間)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate Type日付商品
ZJ24F1216240Certificate of AnalysisJul 10, 2026 Ab211659
ZJ24F1216241Certificate of AnalysisJul 10, 2026 Ab211659
ZJ24F1216242Certificate of AnalysisJul 10, 2026 Ab211659
よくある質問と記事
ソリューション計算機
レビュー

顧客レビュー

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:650nm grade guide → View Em:668nm grade guide →

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