CD45 Mouse mAb - 一次抗体, specific to CD45, Mouse IgG1

Cat. No.: Ab094902

GRADE & PURITY Carrier Free, ExactAb™, Validated, See COA

Application

Flow Cytometry

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

HI30

Conjugation

Unconjugated

概要

Mouse anti Human CD45 Antibody, Monoclonal (HI30), could be used for Flow and so on.
Application:
Flow: < 0.5 μg/100w cells
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Specifications

Product Name

CD45 Mouse mAb - 一次抗体, specific to CD45, Mouse IgG1

同義語

B220 | CD_antigen: CD45 | CD45 antigen | CD45 | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-protein phosphatase C | T

仕様と純度

Carrier Free, ExactAb™, Validated, See COA

宿主種

マウス

特異性

CD45

免疫原

Peripheral blood mononuclear cells

活用

Unconjugated

等級

Carrier Free, ExactAb™, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A purified

集中

See COA

Product Line

biological_products

保管と配送

形状

Liquid

保管条件

Store at 2-8°C

入荷

Wet ice

安定性と保管

Shipped at 4°C. Store at 4°C long term (36 months). Upon receipt, it is recommended to aliquot.

画像

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45 Mouse mAb (Ab094902)

CD45 Mouse mAb (Ab094902) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

Flow

< 0.5µg/100w cells

関連ターゲット（人間）

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD45 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.