CD47 Mouse mAb (Biotin) - 一次抗体, specific to CD47, Mouse IgG1

Cat. No.: Ab185151

グレード＆純度 ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

マウス

Reactive species

人間

Isotype

Mouse IgG1

Clone Number

CC2C6

Conjugation

Biotin

概要

Application:
Flow: ≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

仕様

Product Name

CD47 Mouse mAb (Biotin) - 一次抗体, specific to CD47, Mouse IgG1

同義語

antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 | IAP | IAPintegrin associated protein |

仕様と純度

ExactAb™, Validated, 0.5 mg/mL

宿主種

マウス

特異性

CD47

免疫原

CCRF-CEM T-cell line.

ポジティブコントロール

Flow: MCF-7

活用

Biotin

等級

ExactAb™, Validated

アイソタイプ

Mouse IgG1

ライトチェーン・タイプ

kappa

精製方法

Protein G purified

集中

0.5 mg/mL

Product Line

生物製品

保管と配送

保管条件

Store at 2-8°C,Do not freeze

入荷

Wet ice,Do not freeze

安定性と保管

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

画像

Flow Cytometry: CD47 Mouse mAb (Biotin) (Ab185151)

CD47 Mouse mAb (Biotin) (Ab185151) - Flow Cytometry
Flow cytometry analysis of CD47 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD47 Mouse mAb (Biotin) (Ab185151) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

Flow Cytometry

≤1.0μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. 

関連ターゲット（人間）

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)

Discover Target: CD47

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD47 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.