グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Rabbit anti Human Cdc20 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
仕様
Product Name
Cdc20 Antibody - 一次抗体, specific to Cdc20, Rabbit IgG
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC20.
ポジティブコントロール
WB: A431, THP-1, U-2 OS and C6 cell lysates. IHC: Rat brain tissue.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Rabbit IgG
SDS-PAGE
150 kDa
精製方法
Immunogen affinity purified
集中
1.0 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 20.0 s
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10.0 s
Cdc20 Antibody (Ab095575) - IHC Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Cdc20 Antibody (Ab095575) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ24F0708159
