CDKN3 Antibody - 一次抗体, specific to CDKN3, Rabbit IgG

Cat. No.: Ab095865

グレード＆純度 ExactAb™, Validated, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human CDKN3 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application

WB: 1/500-1/2000
IHC: 1/100-1/300

IF/ICC: 1/200
Protein Function
May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues. Dephosphorylates CDK2 at 'Thr-160' in a cyclin-dependent manner.

仕様

Product Name

CDKN3 Antibody - 一次抗体, specific to CDKN3, Rabbit IgG

同義語

CDI1 antibody | Cdk associated protein phosphatase antibody | CDK2 associated dual specificity phosphatase antibody | CDK2-associated dual-specificity phosphatase antibody | CDKN3 antibody | CDKN3_HUMAN antibody | CIP2 antibody | Cyclin dependent kinase i

仕様と純度

ExactAb™, Validated, 1.0 mg/mL

宿主種

ウサギ

特異性

CDKN3

免疫原

A synthetic peptide derived from human CDKN3 (AA 31-80).

ポジティブコントロール

WB: HEK293, Hep G2, U-2 OS, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue. IF/ICC: HeLa cells.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

1.0 mg/mL

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: CDKN3 Antibody (Ab095865)

CDKN3 Antibody (Ab095865) - Western Blot
All lanes: CDKN3 Antibody (Ab095865) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 18, 24 kDa
Exposure time: 4.5 s

IHC: CDKN3 Antibody (Ab095865)

CDKN3 Antibody (Ab095865) - IHC
Immunohistochemical analysis of paraffin-embedded human Colon cancer. CDKN3 Antibody (Ab095865) was diluted at 1/200 (4° overnight). Tris-EDTA, pH 9.0 was used for antigen retrieval. Secondary antibody was diluted at 1/200 (room temperature, 45 min).

IF/ICC: CDKN3 Antibody (Ab095865)

CDKN3 Antibody (Ab095865) - IF/ICC
IF analysis of CDKN3 Antibody (Ab095865) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with CDKN3 Antibody (Ab095865) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/2000

IF/ICC

1/200

IHC

1/100-1/300

関連ターゲット（人間）

CDKN3 Tbio Cyclin-dependent kinase inhibitor 3 (0 Activities)

Discover Target: CDKN3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)