GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Lyophilized powder?Lyophilized (freeze-dried) powder — gently dried to preserve activity and aid storage. Use for sensitive biologics reconstituted before use.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)≥30 U/mg enzyme powder
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 5 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Cholesterol esterase is an enzyme that catalyzes the hydrolysis of cholesterol esters into free cholesterol and fatty acids, facilitating the absorption of cholesterol in the intestine. Cholesterol esterase commune can be used in combination with cholesterol oxidase to measure cholesterol content.
Enzymatic Properties
Source
Microorganism
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EC Number
EC 3.1.1.13
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Molecular Weight
52 kDa
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pI
5.5
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Km Value
1.9×10⁻⁵ M (Cholesterol linoleate)
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Inhibitors
Not inhibited by sodium azide
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Optimal pH
5.0 ~ 8.5
Figure 1
Optimal Temperature
45°C
Figure 2
pH Stability
4.0 - 9.0
Figure 3
Thermal Stability
Stable below 50°C
Figure 4
附件 Figure 1-Figure 4 放这里
Applications
Used for research and bulk formulation of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) detection reagents.
1.Fangfang Huang, Qingfei Dai, Kewei Zheng, Qingbao Ma, Yu Liu, Shuoqi Jiang, Wei Jiang, Xiaojun Yan. (2023) Exploring the inhibitory potential of KPHs-AL-derived GLLF peptide on pancreatic lipase and cholesterol esterase activities. FOOD CHEMISTRY, [PMID:38061297][10.1016/j.foodchem.2023.138108]
2.Hong Zhang, Wenbin Liu, Lei Wang, Xin Zhang, Bo Wang, Xu Zeng, Bin Zhang. (2024) Functionalized corn stalk carboxymethyl cellulose and mesoporous silica SBA-15 composite hydrogel-immobilized lipase for the resolution of racemic ibuprofen ethyl ester. Green Chemistry Letters and Reviews, [PMID:][10.1080/17518253.2024.2338241]
3.Na Li, Xin Fan, Yefan Wang, Kexin Zhang, Rong Liu, Yunpeng Xu, Zhifeng Tan, Wensi Xu, Dayong Zhou, Deyang Li. (2024) Investigation of isomerization and oxidation of astaxanthin in ready-to-eat Litopenaeus vannamei during accelerated storage. FOOD RESEARCH INTERNATIONAL, [PMID:39277244][10.1016/j.foodres.2024.114983]
4.Tan Min, Mao Jing, Zheng Jianxiong, Meng Yu, Li Jun, Hao Jiayao, Shen Haili. (2024) Mammalian STE20-like kinase 1 inhibits synoviocytes activation in rheumatoid arthritis through mitochondrial dysfunction mediated by SIRT3/mTOR axis. INFLAMMATION RESEARCH, [PMID:38265688][10.1007/s00011-023-01846-5]
5.Liuyi Wei, Taoying Wu, Hao Wu, Li Wen, Bo Cui, Yunhui Cheng. (2026) Unraveling novel pancreatic lipase and cholesterol esterase inhibitory peptides in Oryza Sativa L. and their mechanism by peptidomics, molecular dynamics simulations, and cell study. Food Bioscience, [PMID:][10.1016/j.fbio.2025.108227]
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よくある質問
What does Bioactive mean?
Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does Bioactive mean?
Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
What is the purity of this product, and how is it determined?
This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 9026-00-0.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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