Cl-amidine - 10mM in DMSO , CAS No.1043444-18-3

CAS: 1043444-18-3 Cat. No.: C409028 分子式: C₁₄H₁₉ClN₄O₂.C₂HF₃O₂ 分子量: 424.80

グレード＆純度 10mM in DMSO

Synonyms

Benzamide, N-​[(1S)​-​1-​(aminocarbonyl)​-​4-​[(2-​chloro-​1-​iminoethyl)​amino]​butyl]​-​, 2,​2,​2-​trifluoroacetate (1:1)

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

概要

Information

Cl-amidine Cl-amidine is an irreversible pan- peptidylarginine deiminase (PAD) inhibitor with IC50 values of 5.9 ± 0.3 μM, 0.8 ± 0.3 μM, 6.2 ± 1.0 μM for PAD4, PAD1 and PAD3, respectively. Cl-amidine induces apoptosis .
In vitro

Cl-amidine antagonizes the PAD4-mediated enhancement of the the p300GBD-GRIP1 interaction in a dose-dependent manner. The inhibitory effect of this compound is not a nonspecific one but is targeted at the active PAD4 enzyme. Cl-amidine increases p53 expression in CD45 positive immune cells. It triggers the differentiation and apoptosis of multiple cancer cell lines that are p53+/+ and p53−/− (e.g., HL60, HT29, TK6, and U2-OS cells). Cl-amidine induces the expression of p53 and several downstream target genes including the cyclin dependent kinase inhibitor p21, GADD45, and the proapoptotic protein PUMA in U2-OS osteosarcoma cells.

In vivo

Cl-amidine treatment inhibits NZM(New Zealand mixed 2328) NET(neutrophil extracellular trap) formation in vivo and significantly alters circulating autoantibody profiles and complement levels while reducing glomerular IgG deposition. Further, Cl-amidine increases the differentiation capacity of bone marrow endothelial progenitor cells, improves endothelium-dependent vasorelaxation, and markedly delays time to arterial thrombosis induced by photochemical injury. Cl-amidine delays thrombosis development in NZM mice. It inhibits PADs in mice without significant toxicity and improves disease phenotypes in animal models of inflammatory arthritis and inflammatory bowel disease. And It is shown to reduce disease severity in mouse models of ulcerative colitis and RA.
Cell Data

cell lines：

Concentrations：0-200 μM

Incubation Time：40 h

Powder Purity:≥97%

仕様

同義語

Benzamide, N-​[(1S)​-​1-​(aminocarbonyl)​-​4-​[(2-​chloro-​1-​iminoethyl)​amino]​butyl]​-​, 2,​2,​2-​trifluoroacetate (1:1)

仕様と純度

10mM in DMSO

生化学的・生理学的メカニズム

Cl-amidine is an irreversible pan-peptidylarginine deiminase (PAD) inhibitor with IC50 values of 5.9 ± 0.3 μM, 0.8 ± 0.3 μM, 6.2 ± 1.0 μM for PAD4, PAD1 and PAD3, respectively. Cl-amidine induces apoptosis.

保管条件

Store at -80°C

入荷

Dry ice packs + Cold packs

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

製品の性質

ALogP

1.333

hba_count

3

HBD数

3

回転可能な結合

10

名前と識別子

異性体SMILES

C1=CC=C(C=C1)C(=O)N[C@@H](CCCN=C(CCl)N)C(=O)N.C(=O)(C(F)(F)F)O

代替CAS番号

1373232-26-8；913723-61-2

分子量

424.80

関連ターゲット（人間）

PADI1 Tchem Protein-arginine deiminase type-1 (1 Activities)

Discover Target: PADI1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

化学的性質と物理的性質

• DMSO（mg / mL） 最大溶解度: 84
• DMSO（mM） 最大溶解度: 197.740113
• 水（mg / mL） 最大溶解度: 70
• 水（mM） 最大溶解度: 164.7834275

よくある質問

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1043444-18-3, the molecular formula is C14H19ClN4O2.C2HF3O2, and the molecular weight is 424.80 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.