Creatine Phosphokinase, CAS No.9001-15-4

CAS: 9001-15-4 Cat. No.: L1491908 分子量: 84000

GRADE & PURITY Native, EnzymoPure™, >30 U/mg protein

Accession #

Q9XSC6

Bioactivity

>30 U/mg protein

概要

  Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80 kDa Da. It is made up of 2 subunits, each having a molecular weight of 40 kDa ± 2000. The lighter subunit is present in larger amounts. 
Applications
  Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves. The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.

Specifications

Product Name

Creatine Phosphokinase, CAS No.9001-15-4

同義語

ATP: Creatine N-Phosphotransferase | CPK | Creatine Kinase | Phosphocreatine phosphokinase | CKM | CPK-M | ATP:creatine phosphotransferase | CK | MM-CK | MB-CK | BB-CK | creatine phosphokinase | creatine phosphotransferase | phosphocreatine kinase | adeno

等級

Native, EnzymoPure™

仕様と純度

Native, EnzymoPure™, >30 U/mg protein

生物活性

>30 U/mg protein

アクセス番号

Q9XSC6

予想分子量

80 kDa

CAS

9001-15-4

酵素委員会番号

EC 2.7.3.2

分子タイプ

酵素

Product Line

生物製品

保管と配送

集中

>30 U/mg protein

再構成

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

ユニットの定義

One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.

よくある質問

What does Native mean?

Native indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What is EnzymoPure??

EnzymoPure? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, EnzymoPure? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9001-15-4.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.