CRY2 Mouse mAb - 一次抗体, specific to CRY2

Cat. No.: Ab097865

GRADE & PURITY See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

マウス

Reactive species

しかし, マウス, 人間, 助言, 猿

Isotype

Mouse IgG2a

Clone Number

OTI1H5

Conjugation

Unconjugated

概要

Mouse anti Human CRY2 Antibody, Monoclonal (OTI1H5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

CRY2 Mouse mAb - 一次抗体, specific to CRY2

同義語

cry2 antibody | CRY2_HUMAN antibody | cryptochrome 2 (photolyase like) antibody | Cryptochrome 2 antibody | Cryptochrome-2 antibody | FLJ10332 antibody | growth inhibiting protein 37 antibody | HCRY2 antibody | KIAA0658 antibody | PHLL2 antibody | Photoly

仕様と純度

See COA

生化学的・生理学的メカニズム

Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expres

宿主種

マウス

特異性

CRY2

免疫原

Recombinant full length protein of human CRY2 produced in HEK293T cell.

ポジティブコントロール

WB: Mouse testis lysates. IHC: Human breast adenocarcinoma, colon, colon adenocarcinoma, pancreas carcinoma, thyroid carcinoma, bladder carcinoma, lymphoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY CRY2; HeLa cells. Flow Cytomet

活用

Unconjugated

SDS-PAGE

150 kDa

精製方法

Protein A purified

集中

See COA

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - Western Blot
All lanes: CRY2 Mouse mAb (Ab097865) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution

Predicted band size: 67 kDa
Observed band size: 66 kDa
Exposure time: 100.0 s

IF/ICC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IF/ICC
Immunofluorescent analysis of COS7 cells transiently transfected by pCMV6-ENTRY CRY2.

IF/ICC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IF/ICC
Immunofluorescent analysis of HeLa cells using CRY2 Mouse mAb (Ab097865).

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Adenocarcinoma of Human breast tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon tissue within the normal limits using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Adenocarcinoma of Human colon tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Carcinoma of Human pancreas tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Carcinoma of Human thyroid tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Carcinoma of Human bladder tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - IHC
Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue using CRY2 Mouse mAb (Ab097865). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - Flow Cytometry
Flow cytometric Analysis of HeLa cells, using CRY2 Mouse mAb (Ab097865), (Red), compared to a nonspecific negative control antibody, (Blue).

IHC: CRY2 Mouse mAb (Ab097865)

CRY2 Mouse mAb (Ab097865) - Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using CRY2 Mouse mAb (Ab097865), (Red), compared to a nonspecific negative control antibody, (Blue).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/2000;

• WB:

  1/500;

• IF/ICC:

  1/200;

• IHC:

  1/150

• Flow Cytometry:

  1/200;

関連ターゲット（人間）

CRY2 Tbio Cryptochrome-2 (0 Activities)

Discover Target: CRY2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CRY2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.