DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a

Cat. No.: Ab099443

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IP, WB

Host species

Mouse

Reactive species

Human, Monkey, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

6G8-F4-E3

Conjugation

Unconjugated

Overview

Mouse anti Human DDX3 Antibody, Monoclonal (6G8-F4-E3), could be used for WB, ICC, IF, IP and so on.
Application
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IP: 1/20
Protein Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

Specifications

Product Name

DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a

Synonyms

ATP dependent RNA helicase DDX3X antibody | ATP-dependent RNA helicase DDX3X antibody | CAP Rf antibody | DBX antibody | DDX14 antibody | DDX3X antibody | DDX3X_HUMAN antibody | DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody | DEAD (Asp-Glu-Al

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Mouse

Specificity

DDX3

Immunogen

A synthetic peptide derived from human DDX3 (AA 22-178)

Positive Control

WB: Jurkat, K562, HeLa, A431, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

1.0 mg/mL

Note

This product is about to be sold out and removed from the shelves. The alternative product is Ab099441.

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: DDX3 Mouse mAb (Ab099443)

DDX3 Mouse mAb (Ab099443) - Western Blot
All lanes: DDX3 Mouse mAb (Ab099443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 75 kDa
Exposure time: 18.7 s

IF/ICC: DDX3 Mouse mAb (Ab099443)

DDX3 Mouse mAb (Ab099443) - IF/ICC
Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 Mouse mAb (Ab099443).

IP: DDX3 Mouse mAb (Ab099443)

DDX3 Mouse mAb (Ab099443) - IP
Immunoprecipitation analysis of DDX3 in HeLa lysates using DDX3 Mouse mAb (Ab099443).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IP:

  1/20;

Associated Targets(Human)

DDX3X Tchem ATP-dependent RNA helicase DDX3X (0 Activities)

Discover Target: DDX3X

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action