DNA Loading Buffer (5X, GelRed) - 5X

Cat. No.: R751628

GRADE & PURITY 5X

Synonyms

Red Fluorescent DNA Loading Buffer (5X) | 5xDNA Loading Buffer with GelRed

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

概要

Product Introduction:
GelRed is a novel nucleic acid stain. This unique oil-soluble macromolecule is non-volatile, non-inhalable, and cannot penetrate cell membranes to enter living cells. Additionally, it exhibits no mutagenicity at the gel-staining concentration. GelRed is characterized by its safety in use and high sensitivity in detection. It can be used as a staining agent for various nucleic acid electrophoresis applications and is suitable for staining nucleic acids of all fragment sizes. GelRed is an ideal alternative to ethidium bromide (EB). DNA Loading Buffer (5X, GelRed) needs to be mixed with the sample for staining.
Composition: 10 mM Tris-HCl, 5 mM EDTA, pH 7.6, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, GelRed Nucleic Acid Stain.
Usage Instructions:
1. Use in combination with Aladdin DNA Marker.
2. Mix 4 μl of sample (<500 ng) with 1 μl of DNA Loading Buffer (5X, GelRed). Allow the mixture to stand at room temperature for 2 minutes before loading.
3. It is recommended to use 1.0-2.0% agarose gel, with a voltage of 4-10 V/cm. Perform electrophoresis using 1x TAE (preferred) or 0.5x TBE buffer. Note: Replace the electrophoresis buffer promptly and use freshly prepared gels to achieve optimal results.
4. No additional stains are required in the agarose gel. After electrophoresis, the bands can be directly visualized under UV light. The stain is compatible with commonly used UV transilluminators.

Specifications

同義語

Red Fluorescent DNA Loading Buffer (5X) | 5xDNA Loading Buffer with GelRed

仕様と純度

5X

安定性と保管

Store at 4 ℃ for 36 months. Store at -20 ℃ for 60 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

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Product Line

biological_products