グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Rabbit anti Human DOK7 Antibody, Polyclonal, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.
仕様
Product Name
DOK7 Antibody - 一次抗体, specific to DOK7, Rabbit IgG
同義語
DOK 7 antibody | Docking protein 7 antibody | DOK7 antibody | DOK7_HUMAN antibody | Downstream of tyrosine kinase 7 antibody | Protein Dok-7 antibody | CMS10 | CMS1B | FADS3 | C4orf25
仕様と純度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主種
ウサギ
特異性
DOK7
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DOK7.
ポジティブコントロール
WB: Mouse brain, Rat brain lysates. IHC: Human brain tissue. ICC/IF: Hep G2 cells.
活用
Unconjugated
等級
Carrier Free, ExactAb™, Validated
アイソタイプ
Rabbit IgG
SDS-PAGE
150 kDa
精製方法
Immunogen affinity purified
集中
1.0 mg/mL
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
DOK7 Antibody (Ab100303) - Western Blot All lanes: DOK7 Antibody (Ab100303) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 53 kDa Observed band size: 56 kDa Exposure time: 32.0 s
DOK7 Antibody (Ab100303) - IF/ICC Immunofluorescent analysis of DOK7 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with DOK7 Antibody (Ab100303) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
DOK7 Antibody (Ab100303) - IHC Immunohistochemical analysis of DOK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with DOK7 Antibody (Ab100303) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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