Dulbecco′s Phosphate Buffered Saline, 1X ES Cell Qualified - for cell culture, The Dulbecco's Phosphate Buffered Saline, 1X ES Cell Qualified is available in a 500 mL format & has been optimized & validated for Stem cell culture.

Cat. No.: D475366

GRADE & PURITY for Cell culture, The Dulbecco's Phosphate Buffered Saline, 1X ES Cell Qualified is available in a 500 mL format & has been optimized & validated for Stem cell culture.

Synonyms

D-PBS, DPBS

Storage

Room temperature

概要

Description

Dulbecco′s Phosphate Buffered Saline, 1X ES Cell QualifiedStorage and Stability: Store at 15°C-30°CDisclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.}

Specifications

同義語

D-PBS, DPBS

仕様と純度

for cell culture, The Dulbecco's Phosphate Buffered Saline, 1X ES Cell Qualified is available in a 500 mL format & has been optimized & validated for Stem cell culture.

安定性と保管

Store at 15°C-30°C

法的情報

免责声明: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized c

保管条件

Room temperature

等級

細胞培養用

Application Protocols

No item-specific, validated protocols are provided in the Product Data. Refer to standard cell culture and molecular biology methods for DPBS use.

General example workflows (literature, for illustration only—adjust to your SOPs)

• Cell wash: Aspirate media, add sufficient DPBS to cover cells, gently rock 1–2 times, aspirate; repeat as needed.
• Reagent dilution: Prepare working solutions in DPBS immediately before use; filter-sterilize if sterility is required and the solution permits.
• Tissue handling: Keep samples on ice or at controlled temperature in DPBS; supplement as required for viability (e.g., glucose, buffers like HEPES).

Always verify the formulation (especially Ca2+/Mg2+ and phenol red) on the CoA/label to ensure protocol compatibility for ES cells and other sensitive systems.

Biological Roles

DPBS emulates extracellular ionic conditions and provides phosphate buffering near physiological pH.

General roles (literature)

• Phosphate buffer pair H2PO4−/HPO42− maintains pH ~7.0–7.4, stabilizing protein conformation and cell membrane integrity during manipulations.
• Sodium and potassium ions approximate extracellular ionic strength and support osmotic balance, limiting cell swelling/shrinkage during washing.
• Chloride is the major extracellular anion, balancing cations and maintaining electrochemical neutrality.
• Optional divalent cations (Ca2+, Mg2+)—if present in a given formulation—modulate cell–cell and cell–matrix adhesion, integrin function, and enzyme activities; conversely, their absence can facilitate cell detachment and chelator-based workflows.

For embryonic stem (ES) cells specifically, maintenance of tight control over pH, osmolarity, and trace contaminants is critical; “ES Cell Qualified” buffers are typically vetted in cell culture contexts. For this item, exact qualification criteria and composition are Not specified—review the CoA to confirm suitability for sensitive pluripotent systems.

Buffer Applications

Use cases (general, literature-based)

• Washing adherent or suspension cells, diluting reagents for flow cytometry or microscopy, preparing blanks/controls, and equilibrating materials prior to contact with cells.
• As a vehicle for short-term tissue handling; can be supplemented with nutrients or proteins if required by the protocol.

Typical buffer parameters for 1× DPBS (informational; verify against this item’s CoA)

• pH working range: ~7.0–7.4 at 25 °C.
• Buffering system: phosphate (pKa2 ≈ 7.2), effective near physiological pH.
• Osmolality target: ~300 mOsm/kg.
• Composition variants: with or without Ca2+/Mg2+; with or without phenol red.

Practical guidance

• Choose Ca2+/Mg2+-free DPBS for dissociation and washing steps where reduced adhesion is desired; choose Ca2+/Mg2+-containing DPBS when maintaining junctions/adhesion is beneficial. Composition for SKU D475366 is Not specified—confirm before use.
• Avoid mixing phosphate buffers with solutions that precipitate with phosphate (e.g., certain divalent metal salts) or where phosphate interferes analytically (e.g., some colorimetric assays).
• For long incubations outside CO2 control, consider HEPES-buffered saline instead.

All formulation specifics for D475366 (pH setpoint, osmolarity, ions, indicators) are Not specified; refer to the CoA/Spec Sheet.

Green Alternatives

DPBS is already an aqueous, benign, and nonflammable system. “Greenness” considerations focus on fit-for-purpose selection to minimize waste and rework.

| Option (literature) | Key features | When to choose | Trade-offs | |---|---|---|---| | DPBS (this product) | Phosphate buffer; simple salts | General washing/rinsing; short handling | May be incompatible with phosphate-sensitive assays or precipitation with certain cations | | PBS | Similar to DPBS; some recipes differ slightly in K+/Na+ ratios | Interchangeable in many workflows | Composition nuances can matter for sensitive cells | | HBSS | Contains glucose and bicarbonate (variant-dependent) | Better for prolonged handling or transport of cells | More components; CO2 control may be needed | | HEPES-buffered saline | Strong buffering independent of CO2 | Work outside incubators; imaging | HEPES can generate ROS under light; cost |

Sustainability tips: purchase volumes aligned with usage to reduce expiry waste; store at recommended temperature to extend shelf-life; avoid unnecessary additives; recycle clean plastic where permitted.

Pharmaceutical Uses

This product is designated “for research use only.” It is not validated for human or veterinary applications.

Context (general, literature)

• Phosphate-buffered saline systems are widely used as processing buffers and vehicles in biomanufacturing and analytical QC labs (e.g., chromatography equilibration, dilution of standards). Such uses require GMP-grade materials and lot-specific documentation.

For SKU D475366

• Regulatory status, USP/EP compliance, and excipient grade are Not specified for this item; consult CoA/Spec Sheet if such use is contemplated and ensure materials meet your quality system requirements.

If you need a buffer for regulated manufacturing or clinical applications, select a GMP/excipient-grade PBS/DPBS with full compendial testing and change-control support.

Physical Properties

Item-specific (from Product Data)

• Concentration: 1×, ready to use
• Appearance, pH, osmolality, ionic content, conductivity: Not specified for this item; refer to CoA/Spec Sheet.
• Storage: Room temperature (see Storage & Reconstitution tab)

Typical/literature properties for 1× DPBS (informational, may vary by manufacturer; verify against the item’s CoA)

• pH: ~7.0–7.4 at 25 °C (literature)
• Osmolality: ~270–310 mOsm/kg (literature)
• Density: ~1.00 g/mL at 20–25 °C (approx. water; literature)
• Refractive index: ~1.333 at 20–25 °C (water-like; literature)
• Buffer system: Phosphate (H2PO4−/HPO42−) with pKa2 ≈ 7.2 at 25 °C (literature)
• Boiling/melting point: Dominated by water (BP ~100 °C, MP ~0 °C; literature)
• Solubility: Miscible with water; not applicable for organic solvents (literature)

Notes for practitioners

• Trace metal content, endotoxin level, and mycoplasma/bioburden status are critical for ES cell work but are item-specific; Not specified for this item—consult the CoA/Spec Sheet and SDS for definitive values.
• If calcium/magnesium are present in a given DPBS formulation, they can affect cell adhesion and enzyme dissociation steps; composition must be confirmed prior to use.

Quality and Grades

Item-specific designation

• Grade/Purity: for Cell culture; 1× ES Cell Qualified (from Product Data).

What “for Cell culture” generally entails (literature/industry practice; verify on CoA)

• Produced under controlled conditions for cell culture compatibility, with attention to sterility, particulate control, and low extractables. Often accompanied by lot-specific sterility/endotoxin/mycoplasma testing—these metrics are Not specified for this item; refer to CoA/Spec Sheet.

What “ES Cell Qualified” typically implies (general guidance, not a specification for this lot)

• Qualification against embryonic stem cell workflows (e.g., maintenance of pluripotency markers, normal morphology/adherence during routine handling). Criteria and acceptance ranges vary by supplier and are not standardized; for this item, specifics are Not specified—verify on the CoA or product label.

Stabilizers and additives

• Antibiotics, phenol red, calcium/magnesium salts, or other additives can impact cell behavior. Presence/absence is critical but Not specified for this item; check the CoA/Spec Sheet to match your protocol.

Why this matters

• ES cells are sensitive to buffer composition, osmolarity, and trace contaminants. Use only lots that meet your lab’s acceptance criteria based on the published CoA, and perform small-scale verification upon lot changeover.

Reaction and Applications

This product is intended for life-science workflows rather than chemical synthesis.

Typical applications in cell culture and molecular biology (general guidance)

• Cell washing and resuspension to remove serum, enzymes, or media components without perturbing osmolarity.
• Diluent for water-soluble reagents, antibodies, and staining dyes when divalent cations and proteins are not required (verify Ca2+/Mg2+ status on the CoA for compatibility).
• Maintaining pH near physiological during short manipulations outside CO2-buffered conditions, leveraging the phosphate buffer system.
• Rinsing tissue culture plastics and instruments to establish isotonic conditions prior to cell contact.
• Sample transport buffer for fresh tissues/cell pellets when a simple salt solution is adequate (supplements may be required depending on cell type and duration).

Practical tips

• Warm to room temperature before use to minimize temperature shock to sensitive cells.
• For enzymatic dissociation (e.g., trypsin), many protocols prefer Ca2+/Mg2+-free DPBS to reduce adhesion; verify the item’s formulation before use.
• Avoid prolonged CO2 exposure in open containers; phosphate buffers are minimally affected, but evaporation can shift osmolality.
• Use sterile technique to prevent contamination; once opened, manage by in-use dating and store per the Storage tab.

Reaction Conditions

This product is not intended for chemical synthesis; nevertheless, when used as an aqueous medium for biomolecular manipulations, the following general, literature-based guidance applies:

• Temperature: Room temperature (20–25 °C) is common for washing and short incubations; many cell handling steps also use 37 °C to maintain physiological temperature.
• pH: Governed by the phosphate system; typically ~7.0–7.4 (verify for your lot on the CoA).
• Time: Washes often range from seconds to minutes; longer exposures are protocol dependent.
• Additives: Proteins (e.g., BSA), chelators (e.g., EDTA), or glucose may be added by the user as needed—ensure compatibility with phosphate.
• Compatibility: Avoid systems where phosphate interferes (metal-catalyzed reactions, calcium-dependent assays if Ca2+ is absent/present contrary to needs).

These are general, literature-level conditions, not specifications for SKU D475366. Confirm the actual composition and pH on the CoA before defining critical process parameters.

Safety and Handling

Item-specific hazard information

• Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.

General safety profile (DPBS solutions, literature/guidance)

• Typically considered of low acute hazard; however, not sterile-hazard free—use aseptic technique to prevent contamination of cultures. Not for human or animal therapeutic use.
• Avoid mixing with incompatible materials (strong acids/bases beyond normal buffer range; reactive oxidizers). Do not autoclave sealed containers.

PPE and handling

• Wear standard lab PPE: lab coat, gloves, and safety glasses. Handle under clean conditions in a biosafety cabinet when working with cell culture to maintain sterility.

First aid (general)

• Eye/skin contact: Rinse with water. If irritation persists, seek medical advice.
• Ingestion/inhalation of aerosols: Move to fresh air, rinse mouth; seek medical advice if symptoms occur.

Spill and waste

• Small spills: Wipe with absorbent, then disinfect work surface. Dispose according to institutional policies for nonhazardous aqueous laboratory waste, or biohazard waste if contaminated by biologicals.

Always consult the SDS for SKU D475366 for authoritative safety, transport, and disposal information.

Solvent Selection

Not a solvent for organic synthesis. DPBS is an aqueous, isotonic buffer designed to maintain physiological pH and osmotic balance for cells.

General characteristics (literature)

• Polarity: Highly polar, water-based; dielectric constant ~80 (water at 25 °C).
• Miscibility: Fully miscible with water; not miscible with most nonpolar organic solvents.
• Use case: Washing and diluting mammalian cells/reagents, maintaining cells outside CO2 incubators for short handling periods, and as a vehicle for water-soluble biomolecules.

If you require an organic-compatible buffer or a different ionic composition (e.g., HEPES-buffered saline, HBSS), select based on downstream compatibility, osmolarity, and ion sensitivity of your cells. Verify calcium/magnesium content against your application (enzymatic dissociation, adhesion assays, or chelation-sensitive protocols).

Storage and Reconstitution

Item-specific (from Product Data)

• Storage conditions: Room temperature.
• Format: 1×, ready-to-use solution; no reconstitution required.
• Shipped in: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (literature/practice)

• Protect from microbial contamination; dispense aseptically. If sterility is compromised, discard.
• Avoid prolonged exposure to elevated temperatures and direct sunlight. Do not allow significant evaporation; recap promptly.
• If precipitates form upon cold storage, allow to equilibrate to room temperature and mix to redissolve; persistent precipitation indicates incompatibility/contamination—replace the solution.
• Once opened, assign an in-use shelf life per your laboratory’s quality system. For sterile work, consider aliquoting to minimize repeated container openings.

For any lot-specific shelf life, sterility/endotoxin limits, and composition, consult the CoA/Spec Sheet and SDS for SKU D475366. Research use only.

Structure and Identity

Item-specific (from Product Data)

• Product: Dulbecco′s Phosphate Buffered Saline (DPBS), 1X, ES Cell Qualified (SKU: D475366)
• Intended grade/use: for Cell culture; ES cell qualified
• Format: Aqueous, ready-to-use 1× buffer

What this product is (general description)

• DPBS is a defined, inorganic salt solution in purified water using the phosphate buffering system. It contains sodium and phosphate ions and typically includes potassium ions; optional components in some variants include calcium and magnesium (formulation-dependent; not specified for this item—refer to CoA/Spec Sheet).
• Functional groups: Inorganic salts only; no organic functional groups. No discrete molecular SMILES/InChI apply because it is a mixture/solution.

Identifiers and composition notes

• CAS/EC, SMILES, InChI, InChIKey: Not applicable to a multi-component aqueous buffer; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not applicable as a single entity; Not specified for this item; refer to CoA/Spec Sheet.

2D structural description (general)

• Constituents are simple ions in water: Na+, K+, Cl−, H2PO4−/HPO42− (and, in some formulations, Ca2+, Mg2+). The phosphate conjugate acid/base pair sets pH near physiological. No stereochemistry or ring systems are present.

Synthetic Utility

Not typically used as a reagent in synthetic organic chemistry. However, DPBS can play ancillary roles in aqueous, bioconjugation, or biomolecule-handling workflows.

General utility (literature)

• Aqueous medium for enzyme-catalyzed reactions or bioconjugations where physiological pH and ionic strength are beneficial (e.g., NHS-ester labeling steps performed rapidly to minimize hydrolysis; confirm phosphate compatibility with your chemistry).
• Diluent for nanoparticles, proteins, and nucleic acids during purification, dialysis, or storage where simple salts suffice.
• Reference matrix in analytical method development to simulate physiological ionic strength for chromatography or electrophoresis.

Caveats: Phosphate can coordinate with some metal catalysts and precipitate with certain divalent cations, and it may inhibit reactions requiring low ionic strength or phosphate-free conditions. For nucleophilic acyl substitution chemistries (e.g., NHS-esters), minimize dwell time in aqueous buffers to limit hydrolysis; consider pH and use fresh solutions.

Target Specificity

Not applicable. This product is a buffered saline solution, not a biologic or antibody with antigen specificity. No clone/isotype/species reactivity applies.