E8L Mouse mAb - 一次抗体, specific to E8L

Cat. No.: Ab179942

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Detection Antibody, ELISA

Host species

マウス

Reactive species

ウイルス

Isotype

Mouse IgG2b

Clone Number

GH02/2H12

Conjugation

Unconjugated

概要

Mouse anti MPXV E8L Antibody, clone GH02/2H12, could be used as a detection antibody in ELISA application.

Specifications

Product Name

E8L Mouse mAb - 一次抗体, specific to E8L

同義語

E8L Antibody | Monkeypox Virus E8L Antibody | MPXV E8L Antibody | Anti-Monkeypox virus/MPXV E8L Antibody | Monoclonal Anti-Monkeypox-E8L Antibody | anti-Virus E8L | Monkeypox Virus Cell Surface-Binding Protein (MPXV E8L) Antibody

仕様と純度

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

生化学的・生理学的メカニズム

Binds to chondroitin sulfate on the cell surface to provide virion attachment to target cell.

宿主種

マウス

特異性

E8L

免疫原

Recombinant protein corresponding to MPXV E8L

活用

Unconjugated

等級

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

ライトチェーン・タイプ

kappa

SDS-PAGE

150.0 KDa

精製方法

Protein A purified

マッチドペア

[Ab156143] E8L Mouse mAb, Capture Antibody

集中

1.0 mg/mL

保管と配送

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

画像

Sandwich ELISA - E8L Antibody Pair (Ab156143 & Ab179942)

Sandwich ELISA - E8L standard curve
Sandwich ELISA detection of Recombinant MPXV E8L Protein (rp155937) using E8L Mouse mAb (Ab156143) as capture antibody at concentration of 1.0 µg/mL. Biotinylated E8L Mouse mAb (Ab179942) as detection antibody at concentration of 1.0 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.008 ng/mL - 125.0 ng/mL

Indirect ELISA - E8L Mouse mAb (Ab179942)

Indirect ELISA - E8L standard curve
Immobilized Recombinant MPXV E8L Protein (rp155937) at 1.0 µg/mL overnight at 4℃. Coated protein probed with E8L Mouse mAb (Ab179942). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 5.267 ng/mL

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• ELISA:

  0.5-2000 ng/mL;

• Detection Antibody:

  1.0 μg/mL

よくある質問

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (E8L Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.