Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated ベースライン干渉を最小限に抑える必要がある、感度の高いクロマトグラフィーおよび分析ワークフロー向け。

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保管・配送

Store at 2-8°C,Do not freeze Wet ice,Do not freeze に出荷。正確な仕様については、ロット固有の COA をご確認ください。

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品質書類

SDS、COA、データシート、仕様書をダウンロードできます。ロット固有の COA はロット番号検索で入手可能です。

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文献的裏付け

クロマトグラフィー、有機合成、クロスカップリング反応に関する 0 件の査読済み論文で引用されています。

概要

Mouse anti Human EGFR Antibody, Monoclonal (528), could be used for in vitro EGFR blockade, in vivo EGFR blockade in xenografts, WB, Functional assays, IP, IHC, ICC, IF, Flow and so on.

Application:

in vitro EGFR blockade: Use at an assay dependent concentration.

in vivo EGFR blockade in xenografts: Use at an assay dependent concentration.

WB: Use at an assay dependent concentration.

Functional assays: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

IHC: Use at an assay dependent concentration.

ICC/IF: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.

仕様

Product Name

EGFR Mouse mAb - 一次抗体, specific to EGFR, Mouse IgG2a

同義語

仕様と純度

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

宿主種

マウス

特異性

EGFR

免疫原

Purified EGFR from A431 cells

活用

Unconjugated

等級

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

アイソタイプ

Mouse IgG2a

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at 2-8°C,Do not freeze

入荷

Wet ice,Do not freeze

安定性と保管

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

画像

Flow Cytometry: EGFR Mouse mAb (Ab101169)

EGFR Mouse mAb (Ab101169) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101169) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

適用＃テキヨウ＃

Dilution info