EMSA Probe STAT5 - 1.75μM

Cat. No.: E745619

GRADE & PURITY 1.75μM

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

Aladdin's EMSA Probe – STAT5 is a STAT5 consensus oligonucleotide designed for EMSA . This double-stranded oligonucleotide contains a well-recognized site for STAT5 transcription factors, and can be used as a probe for EMSA. The nucleotide sequence of STAT5 consensus binding oligo is as follows

Precautions：

Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Set up the probe labeling reaction with isotopes by adding reagents in sequence as follows:Probe to be labeled (1.75µM)2µlT4 Polynucleotide Kinase 10X Buffer1µlNuclease-Free Water5µl[γ-32P]ATP (3,000Ci/mmol at 10mCi/ml)1µlT4 Polynucleotide Kinase (5–10u/µl)1µlTotal volume10µl2.To label the probe with biotin, please referred to the EMSA Probe Biotin Labeling Kit (, GS008).3.For cold competition reaction setup, the amount of unlabeled probe should be 50-100 times that of labeled probe.

Specifications

仕様と純度

1.75μM

安定性と保管

Store at -20℃ for up to 1 year.

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。