Dissolve 16.5 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Ammonium Sulfate
1
Potassium Phosphate Dibasic
10.5
Potassium Phosphate Mono
4.5
Sodium Citrate
0.5
Total: 16.5g/Liter
500g makes 32 liters
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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